Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei, Taiwan.
Bone and Joint Research Center, Chang Gung Memorial Hospital, Linko, Taiwan; Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Linko, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110431. doi: 10.1016/j.msec.2019.110431. Epub 2019 Nov 14.
We successfully synthesized a strontium-doped tricalcium silicate (SrCaSiO, Sr = 0 to 2 mol%) bone cement using the sol-gel process. The material properties including crystallinity, setting time, mechanical strength, and hydration products were characterized. Release of ions and pH values of simulated body fluid soaked with the bone cement were measured. In vitro biocompatibility of different concentrations of the material was evaluated by the viability of L929 cells. The setting times of as-prepared slurries were all <70 min. Doping with 0.5 mol% Sr reduced the final setting time by 20 min. After 14 days curing, 0.25 mol% Sr-doped SrCaSiO possessed the highest compressive strength of 45 MPa among all the Sr-doped groups with no statistical difference to CaSiO. The bioactivity of the materials was confirmed with the formation of an apatite layer on the surface of the materials after immersion in simulated body fluid. In addition, the proliferation of L929 cells exposed to 1 mol% Sr was significantly promoted as compared to no Sr doping. SrCaSiO is a novel and advanced material that has the potential to serve as a bone cement in bone restoration with appropriate mechanical strength and favorable biocompatibility.
我们成功地使用溶胶-凝胶法合成了一种锶掺杂的硅酸三钙（SrCaSiO，Sr=0 至 2mol%）骨水泥。对材料的特性包括结晶度、凝固时间、机械强度和水合产物进行了表征。测量了浸泡在骨水泥中的模拟体液中离子的释放和 pH 值。通过 L929 细胞的活力评估了不同浓度材料的体外生物相容性。所制备的浆料的凝固时间均<70min。掺杂 0.5mol% Sr 将最终凝固时间缩短了 20min。14 天固化后，在所有 Sr 掺杂组中，0.25mol% Sr 掺杂 SrCaSiO 的抗压强度最高，达到 45MPa，与 CaSiO 相比无统计学差异。材料的生物活性通过在模拟体液中浸泡后在材料表面形成磷灰石层得到证实。此外，与不掺杂 Sr 相比，暴露于 1mol% Sr 的 L929 细胞的增殖明显增加。SrCaSiO 是一种新型的先进材料，具有作为骨修复用骨水泥的潜力，具有适当的机械强度和良好的生物相容性。
