Department of Food Science, University of Tennessee, Knoxville, TN 37996, USA.
Viruses. 2021 Apr 14;13(4):671. doi: 10.3390/v13040671.
serotype 4b strains are the most prevalent clinical isolates and are widely found in food processing environments. Bacteriophages are natural viral predators of bacteria and are a promising biocontrol agent for . The aims of this study were to characterize phages that specifically infect serotype 4b strains and to assess their ability to inhibit the growth of serotype 4b strains. Out of 120 wild phages, nine phages were selected based on their strong lytic activity against the model serotype 4b strain F2365. These nine phages can be divided into two groups based on their morphological characteristics and host range. Comparison to previously characterized phage genomes revealed one of these groups qualifies to be defined as a novel species. Phages LP-020, LP-027, and LP-094 were selected as representatives of these two groups of phages for further characterization through one-step growth curve and inhibition of serotype 4b experiments. phages that target serotype 4b showed an inhibitory effect on the growth of F2365 and other serotype 4 strains and may be useful for biocontrol of in food processing environments.
4b 血清型菌株是最常见的临床分离株，广泛存在于食品加工环境中。噬菌体是细菌的天然病毒捕食者，是一种有前途的生物防治剂。本研究旨在对特异性感染 4b 血清型菌株的噬菌体进行表征，并评估其抑制 4b 血清型菌株生长的能力。在 120 种野生噬菌体中，根据其对模式 4b 血清型菌株 F2365 的强烈裂解活性，选择了 9 种噬菌体。这 9 种噬菌体可以根据它们的形态特征和宿主范围分为两组。与以前表征的噬菌体基因组的比较表明，其中一组有资格被定义为一个新物种。选择噬菌体 LP-020、LP-027 和 LP-094 作为这两组噬菌体的代表，通过一步生长曲线和抑制 4b 血清型实验进行进一步表征。针对 4b 血清型的噬菌体对 F2365 和其他 4 型菌株的生长表现出抑制作用，可能对食品加工环境中 的生物防治有用。
