Fantini Priscila, Jiménez Román, Vilés Karina, Iborra Antoni, Palhares Maristela Silveira, Catalán Jaime, Prades Marta, Miró Jordi
Department de Medicina i Cirurgia Animal, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.
Departmento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
Animals (Basel). 2021 Oct 9;11(10):2918. doi: 10.3390/ani11102918.
The aim of this study was to standardize a simple, manual platelet-rich plasma (PRP) protocol in Catalonian donkeys using single-spin tube centrifugation as a treatment for jenny endometritis. The objective was to obtain a blood product with a moderate concentration of platelets (2 or 3 times baseline physiologic values) and a low WBC (White Blood Cells) concentration. Blood was drawn from six Catalonian donkeys using acid citrate dextrose (ACD) as an anticoagulant, and then processed by single centrifugation at 133× for two different centrifugation times (10 and 15 min). The PRP samples were evaluated by flow cytometry, and TGF-β1 (Transforming Growth Factor-Beta1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). The 10 min centrifugation protocol resulted in a slightly greater release of TGF-β1 (6044.79 ng/mL), a 2.06-fold increase in platelet concentration, and a 15-fold reduction in leukocyte concentration when compared to the initial values. The 15 min centrifugation time resulted in a 2.44-fold increase in baseline platelet concentration, a reduction in WBC count by a factor of 20, and slightly lower TGF levels (5206 ng/mL). We conclude that both protocols are adequate for the obtention of PRP, and both may have an acceptable therapeutic potential for use in this species, although this needs to be further validated.
本研究的目的是标准化一种简单的手工富血小板血浆（PRP）制备方案，该方案使用单管离心法处理加泰罗尼亚母驴的子宫内膜炎。目标是获得一种血小板浓度适中（为基线生理值的2或3倍）且白细胞（WBC）浓度低的血液制品。使用柠檬酸葡萄糖（ACD）作为抗凝剂，从6头加泰罗尼亚母驴采集血液，然后在133×下进行单次离心，离心时间分别为10分钟和15分钟。通过流式细胞术评估PRP样本，并通过酶联免疫吸附测定（ELISA）测定转化生长因子-β1（TGF-β1）的浓度。与初始值相比，10分钟的离心方案导致TGF-β1释放量略高（6044.79 ng/mL），血小板浓度增加2.06倍，白细胞浓度降低15倍。15分钟的离心时间导致基线血小板浓度增加2.44倍，白细胞计数降低20倍，TGF水平略低（5206 ng/mL）。我们得出结论，两种方案都足以获得PRP，并且两种方案在该物种中可能都具有可接受的治疗潜力，尽管这需要进一步验证。
