State Key Laboratory of Biocontrol, Life Sciences School, Sun Yat-sen University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen University, Guangzhou 510120, China.
Viruses. 2021 Apr 14;13(4):674. doi: 10.3390/v13040674.
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly emerged and highly pathogenic virus that is associated with fatal diarrhea disease in piglets, causing significant economic losses to the pig industry. At present, the research on the pathogenicity and molecular mechanisms of host-virus interactions of SADS-CoV are limited and remain poorly understood. Here, we investigated the global gene expression profiles of SADS-CoV-infected Vero E6 cells at 12, 18, and 24 h post-infection (hpi) using the RNA-sequencing. As a result, a total of 3324 differentially expressed genes (DEG) were identified, most of which showed a down-regulated expression pattern. Functional enrichment analyses indicated that the DEGs are mainly involved in signal transduction, cellular transcription, immune and inflammatory response, and autophagy. Collectively, our results provide insights into the changes in the cellular transcriptome during early infection of SADS-CoV and may provide information for further study of molecular mechanisms.
猪急性腹泻综合征冠状病毒（SADS-CoV）是一种新出现的高致病性病毒，与仔猪致命性腹泻病有关，给养猪业造成了重大经济损失。目前，关于 SADS-CoV 的致病性和宿主-病毒相互作用的分子机制的研究有限，了解甚少。在这里，我们使用 RNA 测序技术研究了感染 SADS-CoV 的 Vero E6 细胞在感染后 12、18 和 24 小时的全基因表达谱。结果共鉴定出 3324 个差异表达基因（DEG），其中大多数呈下调表达模式。功能富集分析表明，DEGs 主要参与信号转导、细胞转录、免疫和炎症反应以及自噬。综上所述，我们的研究结果为 SADS-CoV 早期感染过程中细胞转录组的变化提供了新的见解，为进一步研究分子机制提供了信息。
