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通过核磁共振证明两种不同转运核糖核酸（tRNA）中额外臂与D茎在溶液中的三级相互作用。

Demonstration of a tertiary interaction in solution between the extra arm and the D-stem in two different transfer RNA's by NMR.

作者信息

Salemink P J, Yamane T, Hilbers C W

出版信息

Nucleic Acids Res. 1977 Nov;4(11):3727-41. doi: 10.1093/nar/4.11.3727.

DOI:10.1093/nar/4.11.3727
PMID:339202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343196/
Abstract

According to the X-ray structure of yeast tRNAPhe at 2.5 A resolution, a hydrogen bond is formed between m7G46 and G22. By removal of this m7G46-residue we demonstrate that this interaction is present in solution as well. Comparison of the 1H 360 MHz NMR spectra of intact yeast tRNAPhe and its m7G-excised derivative locates the position of this tertiary H-bond at 12.5 ppm downfield from DSS. Additional evidence for the presence of this interaction in solution comes from a comparison of 1H NMR spectra of E. coli tRNAf1Met and E. coli tRNAf3Met, which differ only in a single position in the extra arm. In tRNAf3Met residue 47 is a m7G-residue, whereas in tRNAf3Met it is A, resulting in the absence of the m7G47 - G23 - C13 triple interaction, characteristic of tRNAf1Met. The resonance position of this tertiary interaction in tRNAf1Met is located around -13.6 ppm, a chemical shift difference of 1.1 ppm with respect to the position observed for tRNAPhe. The origin of this chemical shift difference is discussed in relation to the structure of their respective augmented D-helices.

摘要

根据分辨率为2.5埃的酵母苯丙氨酸转运核糖核酸（tRNAPhe）的X射线结构，在m7G46和G22之间形成了一个氢键。通过去除这个m7G46残基，我们证明这种相互作用在溶液中也存在。完整酵母tRNAPhe及其m7G切除衍生物的1H 360 MHz核磁共振谱的比较确定了这个三级氢键的位置在相对于DSS的场下12.5 ppm处。溶液中存在这种相互作用的额外证据来自对大肠杆菌起始甲硫氨酸转运核糖核酸（tRNAf1Met）和大肠杆菌第三个甲硫氨酸转运核糖核酸（tRNAf3Met）的1H核磁共振谱的比较，它们仅在额外臂的一个位置不同。在tRNAf3Met中，残基47是一个m7G残基，而在tRNAf3Met中它是A，导致缺少tRNAf1Met特有的m7G47 - G23 - C13三重相互作用。tRNAf1Met中这种三级相互作用的共振位置位于约-13.6 ppm处，相对于tRNAPhe观察到的位置有1.1 ppm的化学位移差异。结合它们各自扩展的D螺旋结构讨论了这种化学位移差异的起源。

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