Minkiewicz-Zochniak Anna, Jarzynka Sylwia, Iwańska Agnieszka, Strom Kamila, Iwańczyk Bartłomiej, Bartel Marta, Mazur Maciej, Pietruczuk-Padzik Anna, Konieczna Małgorzata, Augustynowicz-Kopeć Ewa, Olędzka Gabriela
Department of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, Poland.
Department of Microbiology, National Tuberculosis and Lung Diseases Research Institute, Płocka 26, 01-138 Warsaw, Poland.
Materials (Basel). 2021 Apr 17;14(8):2030. doi: 10.3390/ma14082030.
Implants made of ceramic and metallic elements, which are used in dentistry, may either promote or hinder the colonization and adhesion of bacteria to the surface of the biomaterial to varying degrees. The increased interest in the use of dental implants, especially in patients with chronic systemic diseases such as cystic fibrosis (CF), is caused by an increase in disease complications. In this study, we evaluated the differences in the in vitro biofilm formation on the surface of biomaterials commonly used in dentistry (Ti-6Al-4V, cobalt-chromium alloy (CoCr), and zirconia) by isolated from patients with CF. We demonstrated that adherence and growth depends on the type of material used and its surface topography. Weaker bacterial biofilm formation was observed on zirconia surfaces compared to titanium and cobalt-chromium alloy surfaces. Moreover, scanning electron microscopy showed clear differences in bacterial aggregation, depending on the type of biomaterial used. Over the past several decades, strains have developed several mechanisms of resistance, especially in patients on chronic antibiotic treatment such as CF. Therefore, the selection of an appropriate implant biomaterial with limited microorganism adhesion characteristics can affect the occurrence and progression of oral cavity infections, particularly in patients with chronic systemic diseases.
牙科用的由陶瓷和金属元素制成的植入物，可能在不同程度上促进或阻碍细菌在生物材料表面的定植和黏附。对牙科植入物使用的兴趣增加，尤其是在患有慢性全身性疾病如囊性纤维化（CF）的患者中，是由疾病并发症的增加引起的。在本研究中，我们评估了从CF患者中分离出的细菌在牙科常用生物材料（Ti-6Al-4V、钴铬合金（CoCr）和氧化锆）表面形成体外生物膜的差异。我们证明，细菌的黏附和生长取决于所用材料的类型及其表面形貌。与钛和钴铬合金表面相比，在氧化锆表面观察到较弱的细菌生物膜形成。此外，扫描电子显微镜显示，根据所用生物材料的类型，细菌聚集存在明显差异。在过去几十年中，菌株已经产生了几种耐药机制，尤其是在接受慢性抗生素治疗的患者如CF患者中。因此，选择具有有限微生物黏附特性的合适植入生物材料可以影响口腔感染的发生和进展，特别是在患有慢性全身性疾病的患者中。
