School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
Int J Environ Res Public Health. 2021 Apr 15;18(8):4211. doi: 10.3390/ijerph18084211.
Alzheimer's disease (AD) is a neurodegenerative disease that usually affects older individuals. Owing to the higher incidence of root caries and missing teeth in elderly individuals, the bacteria involved in these dental concerns might potentially deteriorate their cognitive function. Altered microbiota in the oral cavity may induce neuroinflammation through migration from the oral cavity to the brain. However, the correlation between the composition of the oral microbiota and neurodegenerative disease remains unclear. In this study, we evaluated sequence to determine the relative abundance and diversity of bacterial taxa in the dental plaque of elderly patients with AD and controls. Oral samples; the DMFT index; and other clinical examination data were collected from 17 patients with AD and 18 normal elderly individuals as the control group. Patients with AD had significantly more missing teeth and higher dental plaque weight but lower microbial diversity than controls. Significantly increased numbers of , , and / and a significantly decreased number of were observed in patients with AD. In conclusion, using the PacBio single-molecule real-time (SMRT) sequencing platform to survey the microbiota dysbiosis biomarkers in the oral cavity of elderly individuals could serve as a tool to identify patients with AD.
阿尔茨海默病（AD）是一种神经退行性疾病，通常发生在老年人身上。由于老年人根龋和缺牙的发病率较高，这些与牙齿相关的细菌可能会潜在地损害他们的认知功能。口腔微生物群的改变可能会通过从口腔向大脑的迁移引发神经炎症。然而，口腔微生物群的组成与神经退行性疾病之间的相关性尚不清楚。在这项研究中，我们评估了序列，以确定 AD 患者和对照组老年人口腔牙菌斑中细菌分类群的相对丰度和多样性。从 17 名 AD 患者和 18 名正常老年个体（对照组）中收集了口腔样本、DMFT 指数和其他临床检查数据。与对照组相比，AD 患者的牙齿缺失和牙菌斑重量明显更高，但微生物多样性较低。AD 患者中观察到显著增加的 、 、 和 / 以及显著减少的 。总之，使用 PacBio 单分子实时（SMRT）测序平台来检测老年人口腔中的微生物失调生物标志物可以作为识别 AD 患者的工具。
