Nørskov Natalja P, Bruhn Annette, Cole Andrew, Nielsen Mette Olaf
Department of Animal Science, Aarhus University, Blichers Alle 20, 8830 Tjele, Denmark.
Center for Circular Bioeconomy, Aarhus University, 8830 Tjele, Denmark.
Metabolites. 2021 Apr 22;11(5):259. doi: 10.3390/metabo11050259.
Greenhouse gas emissions are a global problem facing the dairy/beef industry. Novel feed additives consisting of seaweeds and hemp containing bioactive compounds are theorized to reduce enteric methane emissions. In this study we aimed to investigate the metabolic profiles of brown, red and green seaweeds and hemp using gas chromatography and liquid chromatography mass spectrometry. We used targeted and untargeted approaches, quantifying known halomethanes and phenolics, as well as identifying potentially novel bioactive compounds with anti-methanogenic properties. The main findings were: (a) contained halomethanes, with high concentrations of bromoform (4200 µg/g DW), six volatile halocarbons were tentatively identified; (b) no halomethanes were detected in the other studied seaweeds nor in hemp; (c) high concentrations of lignans were measured in hemp; (d) a high numbers of sulfated phenolic acids and unidentified sulfuric acid-containing compounds were detected in all seaweeds; (e) flavonoid glucosides and glucuronides were mainly identified in hemp; and (f) the condensed tannin gallocatechin was tentatively identified in sp. Using the combined metabolomics approach, an overview and in-depth information on secondary metabolites were provided. Halomethanes of sp. have already been shown to be anti-methanogenic; however, metabolic profiles of seaweeds such as and have also been shown to contain compounds that may have anti-methanogenic potential.
温室气体排放是乳制品/牛肉行业面临的一个全球性问题。由含有生物活性化合物的海藻和大麻组成的新型饲料添加剂被认为可以减少肠道甲烷排放。在本研究中，我们旨在使用气相色谱和液相色谱质谱法研究褐藻、红藻、绿藻和大麻的代谢谱。我们采用了靶向和非靶向方法，对已知的卤代甲烷和酚类进行定量，并鉴定具有抗产甲烷特性的潜在新型生物活性化合物。主要发现如下：(a) 含有卤代甲烷，其中溴仿浓度较高（4200 µg/g干重），初步鉴定出六种挥发性卤代烃；(b) 在其他研究的海藻和大麻中未检测到卤代甲烷；(c) 在大麻中检测到高浓度的木脂素；(d) 在所有海藻中均检测到高含量的硫酸化酚酸和未鉴定的含硫酸化合物；(e) 在大麻中主要鉴定出黄酮糖苷和葡糖醛酸苷；(f) 在 种中初步鉴定出缩合单宁棓儿茶素。使用联合代谢组学方法，提供了关于次生代谢物的概述和深入信息。 种的卤代甲烷已被证明具有抗产甲烷作用；然而， 和 等海藻的代谢谱也已显示含有可能具有抗产甲烷潜力的化合物。
