Wang Anyang, Boroujeni Samaneh Moghadasi, Schneider Philip J, Christie Liam B, Mancuso Kyle A, Andreadis Stelios T, Oh Kwang W
Sensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USA.
Department of Chemical and Biological Engineering, University at Buffalo, State University of New York (SUNY-Buffalo), Buffalo, NY 14260, USA.
Micromachines (Basel). 2021 Apr 23;12(5):482. doi: 10.3390/mi12050482.
We propose an integrated serial dilution generator utilizing centrifugal force with a degassed polydimethylsiloxane (PDMS) microfluidic device. Using gas-soluble PDMS as a centrifugal microfluidic device material, the sample can be dragged in any arbitrary direction using vacuum-driven force, as opposed to in a single direction, without adding further actuation components. The vacuum-driven force allows the device to avoid the formation of air bubbles and exhibit high tolerance in the surface condition. The device was then used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading and sample mixing. In this study, a series of ten-fold serial dilutions ranging from 100 to 10-4 with about 8 μL in each chamber was achieved, while the serial dilution ratio and chamber volume could easily be altered by changing the geometrical designs of the device. As a proof of concept of our hybrid approach with the centrifugal and vacuum-driven forces, ten-fold serial dilutions of a cDNA (complementary DNA) sample were prepared using the device. Then, the diluted samples were collected by fine needles and subject to a quantitative polymerase chain reaction (qPCR), and the results were found to be in good agreement with those for samples prepared by manual pipetting.
我们提出了一种利用离心力的集成式系列稀释发生器，它配备了脱气的聚二甲基硅氧烷（PDMS）微流控装置。使用可溶于气体的PDMS作为离心微流控装置材料，无需添加额外的驱动组件，样品就能利用真空驱动力沿任意方向拖动，而不是单一方向。真空驱动力使该装置能够避免气泡形成，并在表面条件方面表现出高耐受性。然后该装置用于样品计量和样品转移。此外，离心力用于样品加载和样品混合。在本研究中，实现了一系列从100到10⁻⁴的十倍系列稀释，每个腔室约8 μL，同时通过改变装置的几何设计可以轻松改变系列稀释比例和腔室体积。作为我们离心力和真空驱动力混合方法概念验证，使用该装置制备了cDNA（互补DNA）样品的十倍系列稀释液。然后，用细针收集稀释后的样品并进行定量聚合酶链反应（qPCR），结果发现与手动移液制备的样品结果高度一致。
