Choi Yu-Ri, Kim Inyoung, Kumar Manu, Shim Jaekyung, Kim Hyun-Uk
Department of Molecular Biology, Graduate School, Sejong University, Seoul 05006, Korea.
Department of Life Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si 10326, Korea.
Biology (Basel). 2021 Apr 25;10(5):368. doi: 10.3390/biology10050368.
Plants live in ever-changing environments, facing adverse environmental conditions including pathogen infection, herbivore attack, drought, high temperature, low temperature, nutrient deficiency, toxic metal soil contamination, high salt, and osmotic imbalance that inhibit overall plant growth and development. Plants have evolved mechanisms to cope with these stresses. In this study, we found that the () gene in Arabidopsis, which has a lipid-binding FBN domain and a kinase domain, is involved in the plant's response to abiotic stressors, including salt and osmotic stresses. FBN11 protein localizes to the chloroplast. gene expression significantly changed when plants were exposed to the abiotic stress response mediators such as abscisic acid (ABA), sodium chloride (NaCl), and mannitol. The seed germination rates of homozygous mutants in different concentrations of mannitol and NaCl were significantly reduced compared to wild type. ABA-dependent and -independent stress response regulatory genes were differentially expressed in the mutant compared with wild type when grown in mannitol medium. These results suggest a clear role for chloroplast-localized FBN11 in mediating osmotic stress tolerance via the stress response regulatory signaling pathway in the nucleus.
植物生活在不断变化的环境中，面临着各种不利的环境条件，包括病原体感染、食草动物攻击、干旱、高温、低温、营养缺乏、有毒金属土壤污染、高盐和渗透失衡等，这些都会抑制植物的整体生长和发育。植物已经进化出应对这些胁迫的机制。在本研究中，我们发现拟南芥中的（）基因，它具有一个脂质结合FBN结构域和一个激酶结构域，参与植物对非生物胁迫因子的反应，包括盐胁迫和渗透胁迫。FBN11蛋白定位于叶绿体。当植物暴露于脱落酸（ABA）、氯化钠（NaCl）和甘露醇等非生物胁迫反应介质时，该基因的表达发生了显著变化。与野生型相比，不同浓度甘露醇和NaCl处理下的纯合突变体种子萌发率显著降低。在甘露醇培养基中生长时，与野生型相比，突变体中ABA依赖和非依赖的胁迫反应调节基因存在差异表达。这些结果表明，叶绿体定位的FBN11在通过细胞核中的胁迫反应调节信号通路介导渗透胁迫耐受性方面具有明确作用。
