Svensson Fredric G, Manivel Vivek Anand, Seisenbaeva Gulaim A, Kessler Vadim G, Nilsson Bo, Ekdahl Kristina N, Fromell Karin
Department of Molecular Sciences, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden.
Rudbeck Laboratory C5:3, Department of Immunology, Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.
Nanomaterials (Basel). 2021 Apr 24;11(5):1100. doi: 10.3390/nano11051100.
In order to develop a new type of improved wound dressing, we combined the wound healing properties of nanotitania with the advantageous dressing properties of nanocellulose to create three different hybrid materials. The hemocompatibility of the synthesized hybrid materials was evaluated in an in vitro human whole blood model. To our knowledge, this is the first study of the molecular interaction between hybrid nanotitania and blood proteins. Two of the hybrid materials prepared with 3 nm colloidal titania and 10 nm hydrothermally synthesized titania induced strong coagulation and platelet activation but negligible complement activation. Hence, they have great potential as a new dressing for promoting wound healing. Unlike the other two, the third hybrid material using molecular ammonium oxo-lactato titanate as a titania source inhibited platelet consumption, TAT generation, and complement activation, apparently via lowered pH at the surface interface. It is therefore suitable for applications where a passivating surface is desired, such as drug delivery systems and extracorporeal circuits. This opens the possibility for a tailored blood response through the surface functionalization of titania.
为了开发一种新型的改良伤口敷料，我们将纳米二氧化钛的伤口愈合特性与纳米纤维素的有利敷料特性相结合，制备了三种不同的杂化材料。在体外人全血模型中评估了合成杂化材料的血液相容性。据我们所知，这是首次对杂化纳米二氧化钛与血液蛋白之间的分子相互作用进行研究。用3nm胶体二氧化钛和10nm水热合成二氧化钛制备的两种杂化材料诱导了强烈的凝血和血小板活化，但补体活化可忽略不计。因此，它们作为促进伤口愈合的新型敷料具有巨大潜力。与其他两种不同，第三种使用分子氧代乳酸钛铵作为二氧化钛源的杂化材料抑制了血小板消耗、凝血酶-抗凝血酶复合物（TAT）生成和补体活化，显然是通过降低表面界面处的pH值实现的。因此，它适用于需要钝化表面的应用，如药物递送系统和体外循环。这为通过二氧化钛的表面功能化实现定制的血液反应开辟了可能性。
