Beltrami Riccardo, Colombo Marco, Rizzo Keren, Di Cristofaro Alessio, Poggio Claudio, Pietrocola Giampiero
Department of Clinical-Surgical, Diagnostic and Pediatric Sciences-Section of Dentistry, University of Pavia, 27100 Pavia, Italy.
Unit of Biochemestry, Departement of Molecular Medicine, University of Pavia, 27100 Pavia, Italy.
Biomimetics (Basel). 2021 Apr 20;6(2):26. doi: 10.3390/biomimetics6020026.
The aim of the present study was to evaluate and compare the cytotoxic effects of eight composite resins on immortalized human gingival fibroblasts. Composite resins were eluted in cell culture medium for 48 or 72 h at 37 °C. Immortalized human gingival fibroblast-1 (HGF-1) cell lines were seeded in 96-well (1 × 10) plates and incubated for 24 h at 37 °C with the obtained extraction medium. The percentage of viable cells in each well (MTT test) was calculated relative to control cells, which were set to 100%. Data observed were not normally distributed, and nonparametric statistical methods were used for statistical analysis. The Wilcoxon test was used for intragroup comparison, and the Kruskal-Wallis test was used for intergroup multiple comparisons. Significance value was set as < 0.05. All materials tested showed cytotoxic effects on gingival fibroblasts, recordable as noncytotoxic, mildly cytotoxic or severely cytotoxic, depending on the percentage of cell viability. The Wilcoxon test for intragroup comparison showed that the percentage of viable cells decreased significantly for extracts, for all composite resins tested. The composite resins contained monomers that displayed cytotoxic properties. BisGMA, TEGDMA and UDMA had inhibitory effects and induced apoptotic proteins in pulp fibroblast. Composite resins that contained lower percentages of unbound free monomers-and that released less ions-possessed superior biocompatibility in vitro.
本研究的目的是评估和比较八种复合树脂对永生化人牙龈成纤维细胞的细胞毒性作用。复合树脂在37°C的细胞培养基中洗脱48或72小时。将永生化人牙龈成纤维细胞-1（HGF-1）细胞系接种于96孔（1×10）板中，并在37°C下与获得的提取培养基孵育24小时。相对于设定为100%的对照细胞，计算每孔中活细胞的百分比（MTT试验）。观察到的数据呈非正态分布，采用非参数统计方法进行统计分析。Wilcoxon检验用于组内比较，Kruskal-Wallis检验用于组间多重比较。显著性值设定为<0.05。所有测试材料均对牙龈成纤维细胞显示出细胞毒性作用，根据细胞活力百分比可记录为无细胞毒性、轻度细胞毒性或重度细胞毒性。组内比较的Wilcoxon检验表明，对于所有测试的复合树脂，提取物的活细胞百分比显著降低。复合树脂含有具有细胞毒性特性的单体。BisGMA、TEGDMA和UDMA对牙髓成纤维细胞具有抑制作用并诱导凋亡蛋白。未结合游离单体百分比更低且释放离子更少的复合树脂在体外具有更好的生物相容性。
