Faculty of Pharmacy and Nursery, Tay Do University, Can Tho 94000, Vietnam.
Department of Chemistry, University of Education, 280 An Duong Vuong Street, District 5, Ho Chi Minh City 72711, Vietnam.
Molecules. 2021 Apr 13;26(8):2257. doi: 10.3390/molecules26082257.
Bioactive-guided phytochemical investigation of L. growing in Vietnam led to the isolation of five -atisanes, one --atisane, and one lathyrane (ingol-type). The structures were elucidated as -131617-tetrahydroxyatisane (), ethyl -3,4--4,16,17-trihydroxyatisane-3-carboxylate (), -atisane-3-oxo-16,17-acetonide (), -3-acetoxy-16,17-dihydroxyatisane (), -16,17-dihydroxyatisane-3-one (), calliterpenone (), and ingol 12-acetate (). Their chemical structures were unambiguously determined by analysis of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) and high resolution mass spectrometry, as well as by comparison with literature data. Among them, is a new compound while is an ethylated artifact of -3,4--4,16,17-trihydroxyatisane-3-carboxylic acid, a new compound. Isolates were evaluated for alpha-glucosidase inhibition. Compound showed the most significant inhibitory activity against alpha-glucosidase with an IC value of 69.62 µM. Further study on mechanism underlying yeast alpha-glucosidase inhibition indicated that could retard the enzyme function by noncompetitive.
在越南北部采集的 L. 进行生物活性导向的植物化学研究，分离得到了五个 -atisanes、一个 --atisane 和一个 lathyrane（ingol 型）。通过分析一维（1D）和二维（2D）核磁共振（NMR）和高分辨率质谱，以及与文献数据的比较，明确了它们的化学结构。其中，化合物 是一个新化合物，而化合物 是 -3,4--4,16,17-三羟基atisane-3-羧酸的乙基化产物，也是一个新化合物。对分离得到的化合物进行了α-葡萄糖苷酶抑制活性评价，化合物 对α-葡萄糖苷酶的抑制活性最强，IC 值为 69.62µM。对酵母 α-葡萄糖苷酶抑制作用的进一步研究表明，化合物 可以通过非竞争性抑制来延缓酶的功能。
