Nanetti Antonio, Ugolini Luisa, Cilia Giovanni, Pagnotta Eleonora, Malaguti Lorena, Cardaio Ilaria, Matteo Roberto, Lazzeri Luca
Research Centre for Agriculture and Environment (CREA-AA), Council for Agricultural Research and Agricultural Economics Analysis, Via di Saliceto 80, 40128 Bologna, Italy.
Research Centre for Cereal and Industrial Crops (CREA-CI), Council for Agricultural Research and Agricultural Economics Analysis, Via di Corticella 133, 40128 Bologna, Italy.
Microorganisms. 2021 Apr 28;9(5):949. doi: 10.3390/microorganisms9050949.
is a widespread parasite responsible for nosemosis Type C in honey bees, reducing colony survival. The antibiotic fumagillin is the only commercial treatment available, but concerns are emerging about its persistence, safety, and pathogen resistance. The use of natural substances from Brassicaceae defatted seed meals (DSMs) with known antimicrobial and antioxidant properties was explored. Artificially infected bees were fed for 8 days with candies enriched with two concentrations, 2% and 4%, of two DSMs from and , containing a known amount of different glucosinolates (GSLs). The food palatability, GSL intake, bee survival, and treatment effects on spore counts were evaluated. Food consumption was higher for the two 2% DSM patties, for both and , but the GSL intake did not increase by increasing DSM to 4%, due to the resulting lower palatability. The 2% patty decreased the bee mortality, while the higher concentration had a toxic effect. The control was significant for all formulates with respect to the untreated control (312,192.6 +/- 14,443.4 s.e.), and was higher for 4% nigra (120,366.3 +/- 13,307.1 s.e.). GSL hydrolysis products, the isothiocyanates, were detected and quantified in bee gut tissues. Brassicaceae DSMs showed promising results for their nutraceutical and protective effects on bees artificially infected with spores at the laboratory level. Trials in the field should confirm these results.
是一种广泛存在的寄生虫，可导致蜜蜂患C型微孢子虫病，降低蜂群存活率。抗生素腐马霉素是唯一可用的商业治疗药物，但人们对其持久性、安全性和病原体耐药性的担忧日益增加。人们探索了使用具有已知抗菌和抗氧化特性的十字花科脱脂籽粕（DSM）中的天然物质。用富含两种浓度（2%和4%）的来自[具体植物1]和[具体植物2]的两种DSM的糖果喂养人工感染的蜜蜂，这两种DSM含有已知量的不同硫代葡萄糖苷（GSL）。评估了食物适口性、GSL摄入量、蜜蜂存活率以及对[寄生虫名称]孢子计数的治疗效果。对于来自[具体植物1]和[具体植物2]的两种2% DSM肉饼，食物消耗量更高，但由于适口性降低，将DSM浓度提高到4%时，GSL摄入量并未增加。2%的[具体植物1]肉饼降低了蜜蜂死亡率，而较高浓度则产生了毒性作用。与未处理对照（312,192.6 +/- 14,443.4标准误）相比，所有配方的[寄生虫名称]对照均有显著差异，4%的[具体植物2]（黑芥子）对照更高（120,366.3 +/- 13,307.1标准误）。在蜜蜂肠道组织中检测并定量了GSL水解产物异硫氰酸盐。十字花科DSM在实验室水平上对人工感染[寄生虫名称]孢子的蜜蜂具有营养保健和保护作用，显示出有前景的结果。田间试验应证实这些结果。
