Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyusyu 807-8555, Fukuoka, Japan.
Department of Respiratory Medicine, University of Occupational and Environmental Health, Kitakyusyu 807-8555, Fukuoka, Japan.
Int J Mol Sci. 2021 Apr 28;22(9):4635. doi: 10.3390/ijms22094635.
This work studies the relationship between lung inflammation caused by nanomaterials and surfactant protein D (SP-D) kinetics and investigates whether SP-D can be a biomarker of the pulmonary toxicity of nanomaterials. Nanomaterials of nickel oxide and cerium dioxide were classified as having high toxicity, nanomaterials of two types of titanium dioxides and zinc oxide were classified as having low toxicity, and rat biological samples obtained from 3 days to 6 months after intratracheal instillation of those nanomaterials and micron-particles of crystalline silica were used. There were different tendencies of increase between the high- and low-toxicity materials in the concentration of SP-D in bronchoalveolar-lavage fluid (BALF) and serum and in the expression of the SP-D gene in the lung tissue. An analysis of the receiver operating characteristics for the toxicity of the nanomaterials by SP-D in BALF and serum showed a high accuracy of discrimination from 1 week to 3 or 6 months after exposure. These data suggest that the differences in the expression of SP-D in BALF and serum depended on the level of lung inflammation caused by the nanomaterials and that SP-D can be biomarkers for evaluating the pulmonary toxicity of nanomaterials.
本研究探讨了纳米材料引起的肺部炎症与表面活性蛋白 D（SP-D）动力学之间的关系，并探讨了 SP-D 是否可以作为纳米材料肺毒性的生物标志物。将氧化镍和二氧化铈纳米材料归类为高毒性，两种类型的二氧化钛和氧化锌纳米材料归类为低毒性，使用大鼠生物样本，这些纳米材料和结晶二氧化硅的微米颗粒在气管内滴注后 3 天至 6 个月。在支气管肺泡灌洗液（BALF）和血清中 SP-D 的浓度以及肺组织中 SP-D 基因的表达方面，高毒性和低毒性材料之间呈不同的升高趋势。通过 BALF 和血清中的 SP-D 对纳米材料毒性的受试者工作特征分析表明，在暴露后 1 周至 3 或 6 个月时，其区分毒性的准确性很高。这些数据表明，BALF 和血清中 SP-D 的表达差异取决于纳米材料引起的肺部炎症程度，并且 SP-D 可以作为评估纳米材料肺毒性的生物标志物。
