Li Xiaoshuai, Wang Jia, Zhang Huayue, Xiao Long, Lei Zhongfang, Kaul Sunil C, Wadhwa Renu, Zhang Zhenya
Graduate School of Life & Environmental Science, University of Tsukuba, Ibaraki 305-8575, Japan.
AIST-INDIA DAILAB, National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan.
J Fungi (Basel). 2021 Apr 28;7(5):342. doi: 10.3390/jof7050342.
possesses several compounds with medicinal properties, and is commonly used in traditional Chinese functional food and medicine for a variety of health benefits. Because of its rare occurrence in nature, the market demand for artificial is on the rise. Furthermore, efforts to increase its bioactive ingredients have also been considered in research. In this study, we aimed to investigate the effect of fluoride on the growth and enrichment of bioactive compounds in . A wide range of potassium fluoride concentrations (0, 0.001, 0.01, 0.1, and 1 mM) were added to the culture media as a source of fluoride during the cultivation of fruiting bodies. The contents of fluorine and bioactive substances of the fruiting bodies in normal (NM) and fluorine-supplemented (FM) media were measured and compared. raised in growth medium supplemented with 0.01 mM potassium fluoride led to a 44.86% (1.55 ± 0.14 g/bottle) increase in biomass and a 23.43% (3161.38 ± 35.71 µg/g) increase in total carotenoid content in the fruiting bodies. Furthermore, a remarkable increase in superoxide dismutase-like activity (84.75 U/mg) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC = 2.59 mg/mL) was recorded. In human cancer cell-based assays, raised in FM caused stronger cytotoxicity, apoptosis, and cell cycle arrest in human osteosarcoma cells. These results demonstrated that a low dose of fluoride could stimulate the growth of fruiting bodies and enhance the production of bioactive ingredients that possess useful antioxidant and anticancer activities.
含有多种具有药用特性的化合物，常用于传统的中国功能性食品和药物中，具有多种健康益处。由于其在自然界中罕见，人工合成的市场需求正在上升。此外，研究中也考虑了增加其生物活性成分的努力。在本研究中，我们旨在研究氟化物对[具体名称]中生物活性化合物生长和富集的影响。在[具体名称]子实体培养过程中，向培养基中添加了一系列不同浓度的氟化钾（0、0.001、0.01、0.1和1 mM）作为氟源。测量并比较了正常（NM）培养基和添加氟（FM）培养基中[具体名称]子实体的氟含量和生物活性物质含量。在添加0.01 mM氟化钾的[具体名称]生长培养基中培养的[具体名称]，其生物量增加了44.86%（1.55±0.14 g/瓶），子实体中总类胡萝卜素含量增加了23.43%（3161.38±35.71 µg/g）。此外，超氧化物歧化酶样活性（84.75 U/mg）和2,2-二苯基-1-苦基肼自由基清除活性（IC = 2.59 mg/mL）有显著增加。在基于人类癌细胞的试验中，在FM中培养的[具体名称]对人骨肉瘤细胞具有更强的细胞毒性作用、诱导凋亡作用和细胞周期阻滞作用。这些结果表明，低剂量的氟化物可以刺激[具体名称]子实体的生长，并增强具有有用抗氧化和抗癌活性的生物活性成分的产生。
