Fujiwara Aki, Tsukada Mana, Ikemoto Hideshi, Izuno Takuji, Hattori Satoshi, Okumo Takayuki, Hisamitsu Tadashi, Sunagawa Masataka
Department of Physiology, School of Medicine, Showa University, Tokyo 142-8555, Japan.
Acupuncture & Moxibustion Clinic Tenshinotamago, Tokyo 104-0061, Japan.
Healthcare (Basel). 2021 Apr 27;9(5):503. doi: 10.3390/healthcare9050503.
The aim of this research was to investigate the antistress effect of press tack needle (PTN) acupuncture treatment using rats with social isolation stress (SIS). Rats were divided into non-stress group (Grouped+sham), stress group (SIS+sham), and PTN-treated SIS group (SIS+PTN). Rats in the SIS+PTN and SIS+sham groups were housed alone for eight days. For the SIS+PTN group, a PTN (length, 0.3 or 1.2 mm) was fixed on the GV20 acupoint on day 7. We measured stress behavior based on the time the rats showed aggressive behavior and the levels of plasma corticosterone and orexin A on day 8. In addition, the orexin-1 receptor or orexin-2 receptor antagonist was administered to rats that were exposed to SIS. The duration of aggressive behavior was significantly prolonged in the SIS+sham group, and the prolonged duration was inhibited in the SIS+PTN (1.2 mm) group. The levels of plasma corticosterone and orexin A were significantly increased in the SIS+sham group; however, these increases were inhibited in the SIS+PTN group. The aggressive behavior was significantly reduced after the orexin-2 receptor antagonist was administered. These findings suggest that PTN treatment at GV20 may have an antistress effect, and the control of orexin is a mechanism underlying this phenomenon.
本研究旨在探讨揿针针刺治疗对社会隔离应激（SIS）大鼠的抗应激作用。将大鼠分为非应激组（群居+假针刺）、应激组（SIS+假针刺）和经揿针治疗的SIS组（SIS+揿针）。SIS+揿针组和SIS+假针刺组的大鼠单独饲养8天。对于SIS+揿针组，在第7天将一根揿针（长度为0.3或1.2毫米）固定在GV20穴位上。我们在第8天根据大鼠出现攻击行为的时间以及血浆皮质酮和食欲素A的水平来测量应激行为。此外，将食欲素-1受体或食欲素-2受体拮抗剂给予遭受SIS的大鼠。SIS+假针刺组的攻击行为持续时间显著延长，而在SIS+揿针（1.2毫米）组中，这种延长的持续时间受到抑制。SIS+假针刺组的血浆皮质酮和食欲素A水平显著升高；然而，这些升高在SIS+揿针组中受到抑制。给予食欲素-2受体拮抗剂后，攻击行为显著减少。这些发现表明，在GV20进行揿针治疗可能具有抗应激作用，而对食欲素的调控是这一现象的潜在机制。
