Nishiyama Masateru, Praise Susan, Tsurumaki Keiichi, Baba Hiroaki, Kanamori Hajime, Watanabe Toru
Department of Food, Life and Environmental Sciences, Faculty of Agriculture, Yamagata University, Tsuruoka 9978555, Japan.
Department of Infectious Diseases, Internal Medicine, Tohoku University Graduate School of Medicine, Sendai 9808574, Japan.
Antibiotics (Basel). 2021 Apr 26;10(5):495. doi: 10.3390/antibiotics10050495.
There is increasing attention toward factors that potentially contribute to antibiotic resistance (AR), as well as an interest in exploring the emergence and occurrence of antibiotic resistance bacteria (ARB). We monitored six ARBs that cause hospital outbreaks in wastewater influent to highlight the presence of these ARBs in the general population. We analyzed wastewater samples from a municipal wastewater treatment plant (MWWTP) and hospital wastewater (HW) for six species of ARB: Carbapenem-resistant Enterobacteria (CARBA), extended-spectrum β-lactamase producing Enterobacteria (ESBL), multidrug-resistant Acinetobacter (MDRA), multidrug-resistant (MDRP), methicillin-resistant (MRSA), and vancomycin-resistant Enterococci (VRE). We registered a high percentage of ARBs in MWWTP samples (>66%) for all ARBs except for MDRP, indicating a high prevalence in the population. Percentages in HW samples were low (<78%), and no VRE was detected throughout the study. CARBA and ESBL were detected in all wastewater samples, whereas MDRA and MRSA had a high abundance. This result demonstrated the functionality of using raw wastewater at MWWTP to monitor the presence and extent of ARB in healthy populations. This kind of surveillance will contribute to strengthening the efforts toward reducing ARBs through the detection of ARBs to which the general population is exposed.
人们越来越关注可能导致抗生素耐药性（AR）的因素，同时也对探索抗生素耐药菌（ARB）的出现和发生情况感兴趣。我们监测了六种在医院污水流入中引发医院感染爆发的ARB，以突显这些ARB在普通人群中的存在情况。我们分析了来自城市污水处理厂（MWWTP）的污水样本和医院污水（HW）中的六种ARB：耐碳青霉烯类肠杆菌（CARBA）、产超广谱β-内酰胺酶肠杆菌（ESBL）、多重耐药不动杆菌（MDRA）、多重耐药铜绿假单胞菌（MDRP）、耐甲氧西林金黄色葡萄球菌（MRSA）和耐万古霉素肠球菌（VRE）。除MDRP外，我们在MWWTP样本中检测到所有ARB的比例都很高（>66%），这表明在人群中具有高流行率。HW样本中的比例较低（<78%），并且在整个研究过程中未检测到VRE。在所有污水样本中均检测到CARBA和ESBL，而MDRA和MRSA的丰度较高。这一结果证明了利用MWWTP的原污水监测健康人群中ARB的存在情况及其程度的可行性。这种监测将有助于通过检测普通人群接触到的ARB来加强减少ARB的工作。
