Kazlauskaite Jurga Andreja, Ivanauskas Liudas, Bernatoniene Jurga
Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.
Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.
Pharmaceutics. 2021 Apr 26;13(5):620. doi: 10.3390/pharmaceutics13050620.
L. is receiving increasing attention due to the isoflavones it contains, which have been studied for their benefits to human health. A common problem with isoflavone aglycones is a rather low water solubility and limited pharmaceutical applications. The use of excipients, such as cyclodextrins in the production of isoflavone rich extracts, could become one of the new strategies for the extraction of target compounds. The aim of this study was to evaluate an eco-friendly method using the effects of -, - and -cyclodextrins for isoflavone solubilization in plant extracts in comparison to a standard extract without excipients. Extractions of red clover were prepared using ultrasound-assisted combined with thermal hydrolysis and heat reflux. It was determined that cyclodextrins significantly increased the isoflavones aglycone yields. By increasing cyclodextrins in the extraction media from 1 to 5%, the daidzin concentration increased on average by 1.06 (-cyclodextrins), 1.4 (-cyclodextrins) and 1.25 (-cyclodextrins) times. Genistein concentration increased using - and -cyclodextrins (1.28 and 1.12 times, - and -cyclodextrins, respectively), but decreased using -cyclodextrins. The results showed that the cyclodextrin-assisted extraction enhanced the yields of isoflavones from red clover, which suggests using cyclodextrins as a green alternative and a cost-effective method to increase its pharmaceutical application.
由于其所含的异黄酮，红三叶草受到越来越多的关注，这些异黄酮对人体健康的益处已得到研究。异黄酮苷元的一个常见问题是水溶性相当低且药物应用有限。在富含异黄酮提取物的生产中使用辅料，如环糊精，可能成为提取目标化合物的新策略之一。本研究的目的是评估一种环保方法，即使用α-、β-和γ-环糊精对植物提取物中的异黄酮进行增溶，并与不含辅料的标准提取物进行比较。采用超声辅助结合热水解和热回流的方法制备红三叶草提取物。结果表明，环糊精显著提高了异黄酮苷元的产量。通过将提取介质中环糊精的含量从1%提高到5%，大豆苷浓度平均分别增加了1.06倍（α-环糊精）、1.4倍（β-环糊精）和1.25倍（γ-环糊精）。使用β-和γ-环糊精时染料木黄酮浓度增加（分别为1.28倍和1.12倍，β-和γ-环糊精），但使用α-环糊精时浓度降低。结果表明，环糊精辅助提取提高了红三叶草中异黄酮的产量，这表明使用环糊精作为一种绿色替代方法和一种经济有效的方法来增加其药物应用。
