Programa de Pós-Graduação em Biotecnologia, Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, CEP 85660-000 Dois Vizinhos, Paraná, Brazil.
Departamento de Química, Universidade Tecnológica Federal do Paraná, Campus Pato Branco, CEP 85503-390 Pato Branco, Paraná, Brazil.
Int J Biol Macromol. 2021 Jul 31;183:316-330. doi: 10.1016/j.ijbiomac.2021.04.169. Epub 2021 Apr 27.
A hydrogel containing exocellular (1 → 6)-β-D-glucan (lasiodiplodan, LAS) was developed and its wound healing potential was evaluated. β-Glucans have attracted much interest by the cosmetic industry sector because of their bioactive and functional properties and in promoting skin health. In the present work an β-glucan was studied as a healing biomaterial that has not hitherto been reported in the scientific literature. LAS produced by the ascomycete Lasiodiplodia theobromae MMPI was used in the formulation of a healing hydrogel. Physicochemical and microbiological quality parameters, antioxidant potential and stability of the formulation was evaluated. FTIR, thermal analysis and SEM techniques were also employed in the characterization. Wistar rats were used as a biological model to investigate the wound healing potential. Histological analyses of cutaneous tissue from the dorsal region were conducted after 4, 7, 10 and 14 days of treatment, and evaluated re-epithelialization, cell proliferation and collagen production. Physicochemical stability, microbiological quality and antioxidant potential, especially in relation to its ability to scavenge hydroxyl radicals were found. The hydrogel stimulated cell re-epithelialization and proliferation during all days of the treatment, and stimulated an increase of collagen fibers. Lasiodiplodan showed immunomodulatory activity in wound healing and this biomacromolecule could be an alternative compound in wound care.
一种含有胞外（1→6）-β-D-葡聚糖（拉索胶，LAS）的水凝胶被开发出来，并评估了其伤口愈合的潜力。β-葡聚糖因其生物活性和功能特性以及促进皮肤健康而受到化妆品行业的广泛关注。在本工作中，研究了一种作为愈合生物材料的β-葡聚糖，这在科学文献中尚未有报道。本研究使用由曲霉菌 Lasiodiplodia theobromae MMPI 产生的 LAS 来制备一种水凝胶。评估了制剂的物理化学和微生物质量参数、抗氧化潜力和稳定性。还采用了傅里叶变换红外光谱（FTIR）、热分析和扫描电子显微镜（SEM）技术进行了表征。使用 Wistar 大鼠作为生物模型来研究伤口愈合的潜力。在治疗后 4、7、10 和 14 天对背部皮肤组织进行了组织学分析，并评估了再上皮化、细胞增殖和胶原生成。发现该水凝胶具有物理化学稳定性、微生物质量和抗氧化潜力，尤其是其清除羟自由基的能力。水凝胶在整个治疗过程中刺激细胞再上皮化和增殖，并刺激胶原纤维的增加。拉索胶在伤口愈合中表现出免疫调节活性，这种生物大分子可能是伤口护理的一种替代化合物。
