解析假单胞菌 SHC-8-1 O-抗原的结构并注释其基因簇，该 O-抗原含有 2-乙酰氨基-2,4,6-三脱氧-4-(3,5-二羟基己酰氨基)-d-葡萄糖。
Structure elucidation and gene cluster annotation of the O-antigen of Pseudomonas veronii SHC-8-1 containing 2-acetamido-2,4,6-trideoxy-4-(3,5-dihydroxyhexanoylamino)-d-glucose.
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119334, Russia.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119334, Russia.
出版信息
Carbohydr Res. 2021 Jun;504:108306. doi: 10.1016/j.carres.2021.108306. Epub 2021 Apr 5.
O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-trideoxy-dglucose (Bacillosamine) in which N-2 is acetylated and N-4 is acylated with 3,5-dihydroxyhexanoic acid (dHh). The O-antigen gene cluster of Pseudomonas veronii SHC-8-1 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure.
O-多糖（O-抗原，OPS）从假单胞菌 veronii SHC-8-1 的脂多糖中分离出来，并通过成分分析和 1D 和 2D NMR 光谱进行了研究。O-多糖的结构如下：其中 QuipNAc4N(dHh)是 2,4-二氨基-2,4,6-三脱氧-dglucose（Bacillosamine），其中 N-2 乙酰化，N-4 与 3,5-二羟基己酸（dHh）酰化。假单胞菌 veronii SHC-8-1 的 O-抗原基因簇已被测序。通过与现有数据库中的序列进行比较，推测了基因的功能，并与 OPS 结构一致。
Zotero 插件