Wang Pengjie, Jin Shan, Chen Xuejin, Wu Liangyu, Zheng Yucheng, Yue Chuan, Guo Yongchun, Zhang Xingtan, Yang Jiangfan, Ye Naixing
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, 350002, China.
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Hortic Res. 2021 May 1;8(1):96. doi: 10.1038/s41438-021-00529-8.
Plants have evolved regulatory mechanisms at multiple levels to regulate gene expression in order to improve their cold adaptability. However, limited information is available regarding the stress response at the chromatin and translational levels. Here, we characterize the chromatin accessibility, transcriptional, and translational landscapes of tea plants in vivo under chilling stress for the first time. Chilling stress significantly affected both the transcription and translation levels as well as the translation efficiency of tea plants. A total of 3010 genes that underwent rapid and independent translation under chilling stress were observed, and they were significantly enriched in the photosynthesis-antenna protein and phenylpropanoid biosynthesis pathways. A set of genes that were significantly responsive to cold at the transcription and translation levels, including four (+)-neomenthol dehydrogenases (MNDs) and two (E)-nerolidol synthases (NESs) arranged in tandem on the chromosomes, were also found. We detected potential upstream open reading frames (uORFs) on 3082 genes and found that tea plants may inhibit the overall expression of genes by enhancing the translation of uORFs under chilling stress. In addition, we identified distal transposase hypersensitive sites (THSs) and proximal THSs and constructed a transcriptional regulatory network for tea plants under chilling stress. We also identified 13 high-confidence transcription factors (TFs) that may play a crucial role in cold regulation. These results provide valuable information regarding the potential transcriptional regulatory network in plants and help to clarify how plants exhibit flexible responses to chilling stress.
植物已经进化出多层次的调控机制来调节基因表达，以提高其冷适应性。然而，关于染色质和翻译水平上的应激反应的信息有限。在这里，我们首次对低温胁迫下茶树体内的染色质可及性、转录和翻译情况进行了表征。低温胁迫显著影响了茶树的转录和翻译水平以及翻译效率。共观察到3010个基因在低温胁迫下经历了快速且独立的翻译，它们在光合天线蛋白和苯丙烷生物合成途径中显著富集。还发现了一组在转录和翻译水平上对低温有显著响应的基因，包括四个在染色体上串联排列的(+)-新薄荷醇脱氢酶（MNDs）和两个(E)-橙花叔醇合酶（NESs）。我们在3082个基因上检测到潜在的上游开放阅读框（uORFs），并发现茶树可能在低温胁迫下通过增强uORFs的翻译来抑制基因的整体表达。此外，我们鉴定了远端转座酶超敏位点（THSs）和近端THSs，并构建了低温胁迫下茶树的转录调控网络。我们还鉴定了13个可能在低温调控中起关键作用的高可信度转录因子（TFs）。这些结果为植物潜在的转录调控网络提供了有价值的信息，并有助于阐明植物如何对低温胁迫表现出灵活的反应。
