Department of Biology, Faculty of Basic Sciences, Shahrekord University, Sharekord, Iran.
Department of Biology, Faculty of Basic Sciences, Shahrekord University, Sharekord, Iran; Biotechnology Research Institute, Shahrekord University, Shahrekord, Iran.
Colloids Surf B Biointerfaces. 2021 Aug;204:111774. doi: 10.1016/j.colsurfb.2021.111774. Epub 2021 Apr 19.
A variety of organic nanomaterials and organic polymers are used for enzyme immobilization to increase enzymes stability and reusability. In this study, the effects of the immobilization of enzymes on organic and organic-inorganic hybrid nano-supports are compared. Immobilization of enzymes on organic support nanomaterials was reported to significantly improve thermal, pH and storage stability, acting also as a protection against metal ions inhibitory effects. In particular, the effects of enzyme immobilization on reusability, physical, kinetic and thermodynamic parameters were considered. Due to their biocompatibility with low health risks, organic support nanomaterials represent a good choice for the immobilization of enzymes. Organic nanomaterials, and especially organic-inorganic hybrids, can significantly improve the kinetic and thermodynamic parameters of immobilized enzymes compared to macroscopic supports. Moreover, organic nanomaterials are more environment friendly for medical applications, such as prodrug carriers and biosensors. Overall, organic hybrid nanomaterials are receiving increasing attention as novel nano-supports for enzyme immobilization and will be used extensively.
各种有机纳米材料和有机聚合物被用于酶固定化以提高酶的稳定性和可重复使用性。在这项研究中，比较了酶在有机和有机-无机杂化纳米载体上的固定化效果。酶在有机纳米材料载体上的固定化被报道可以显著提高热稳定性、pH 稳定性和储存稳定性，同时还可以防止金属离子的抑制作用。特别是，考虑了酶固定化对可重复使用性、物理、动力学和热力学参数的影响。由于其与低健康风险的生物相容性，有机纳米材料载体是酶固定化的一个很好的选择。与宏观载体相比，有机纳米材料，尤其是有机-无机杂化纳米材料，可以显著改善固定化酶的动力学和热力学参数。此外，有机纳米材料在药物载体和生物传感器等医学应用中更具环境友好性。总的来说，有机杂化纳米材料作为新型酶固定化纳米载体受到越来越多的关注，并将得到广泛应用。
