一种简单且低成本的 Resazurin assay 方法，可用于评估跨物种的活力。

A simple and low-cost resazurin assay for vitality assessment across species.

机构信息

Chair of Bioprocess Engineering, University of Kaiserslautern, 67663 Kaiserslautern, Germany.

出版信息

J Biotechnol. 2021 Jun 10;333:63-66. doi: 10.1016/j.jbiotec.2021.04.010. Epub 2021 Apr 30.

DOI:10.1016/j.jbiotec.2021.04.010

Abstract

Working with biological organisms requires knowledge about the state of their viability and vitality to ascertain efficient processes. The phenoxazine dye resazurin is routinely used for viability assessment of many different species. Here, a novel use for resazurin as an indicator for vitality assessment across several species is proposed. Different amounts of biomass as well as mixtures of live/dead biomass were investigated for their capabilities of metabolizing resazurin and monitored over time. Increasing (live) biomass was found to increase reaction rate in a linear fashion, giving information about the cells' vitality. In an application example, stored suspension cultures of Sporosarcina pasteurii were found to decrease in viability over time, while urease activity decreased as well. For the first time, the assessment of vitality by one technique was demonstrated for several species in parallel.

摘要

与生物有机体合作需要了解其生存能力和活力的状态，以确定有效的过程。苯并嗪染料 Resazurin 通常用于评估许多不同物种的生存能力。在这里，提出了 Resazurin 作为几种物种活力评估指标的新用途。研究了不同量的生物量以及活/死生物量的混合物，以考察它们代谢 Resazurin 的能力，并随时间进行监测。发现增加（活）生物量会以线性方式增加反应速率，从而提供有关细胞活力的信息。在一个应用示例中，发现储存的巴氏芽孢杆菌悬浮培养物的生存能力随着时间的推移而降低，而脲酶活性也降低了。这是首次同时对几种物种进行了一种技术的活力评估。