Mendes Rafael J, Sario Sara, Luz João Pedro, Tassi Natália, Teixeira Cátia, Gomes Paula, Tavares Fernando, Santos Conceição
Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal.
LAQV-REQUIMTE, Biology Department, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal.
Plants (Basel). 2021 Nov 30;10(12):2637. doi: 10.3390/plants10122637.
Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees. This disease is caused by a quarantine phytopathogenic bacterium, which, although highly distributed worldwide, still lacks efficient control measures. The green revolution paradigm demands sustainable agriculture practices, for which antimicrobial peptides (AMPs) have recently caught much attention. The goal of this work was to disclose the bioactivity of three peptides mixtures (BP100:RW-BP100, BP100:CA-M, and RW-BP100:CA-M), against three strains of representing distinct genotypes and virulence (LMG 2024, Ea 630 and Ea 680). The three AMPs' mixtures were assayed at eight different equimolar concentrations ranging from 0.25 to 6 μM (1:1). Results showed MIC and MBC values between 2.5 and 4 μM for every AMP mixture and strain. Regarding cell viability, flow cytometry and alamarBlue reduction, showed high reduction (>25%) of viable cells after 30 min of AMP exposure, depending on the peptide mixture and strain assayed. Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant. Altogether, the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually.
火疫病是一种严重的细菌性植物病害，会影响梨树和苹果树等重要的具有经济价值的果树。这种病害由一种检疫性植物病原细菌引起，尽管该细菌在全球广泛分布，但仍缺乏有效的防治措施。绿色革命模式要求采用可持续农业做法，为此抗菌肽（AMPs）最近备受关注。这项工作的目标是揭示三种肽混合物（BP100:RW - BP100、BP100:CA - M和RW - BP100:CA - M）对代表不同基因型和毒力的三种菌株（LMG 2024、Ea 630和Ea 680）的生物活性。这三种抗菌肽混合物在0.25至6 μM（1:1）的八种不同等摩尔浓度下进行了检测。结果显示，每种抗菌肽混合物和菌株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值在2.5至4 μM之间。关于细胞活力，流式细胞术和alamarBlue还原实验表明，根据所检测的肽混合物和菌株，抗菌肽暴露30分钟后活细胞数量大幅减少（>25%）。烟草植株中的过敏反应表明，最有效的抗菌肽混合物及其浓度对植株造成的反应很小或没有反应。总体而言，与单独应用相同的抗菌肽相比，所研究的抗菌肽混合物是控制火疫病更好的治疗方案。
