Rizwana Humaira, Bokahri Najat A, Alsahli Sarah A, Al Showiman Amal S, Alzahrani Rawan M, Aldehaish Horiah A
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Saudi J Biol Sci. 2021 Apr;28(4):2236-2244. doi: 10.1016/j.sjbs.2021.01.014. Epub 2021 Jan 23.
The aim of this study was to assess L. fruit extracts as an alternative to synthetic fungicide against (Fries) Keissler, the causative agent of black spots of tomato fruit. Antifungal activities of pulp and seed extracts were tested both and The seed extracts were more potent at inhibiting than the pulp extracts. The assay showed maximum inhibition of radial mycelial growth of (90%) by methanol seed extracts, at the highest concentration of 6%. Similarly, the assay showed marked reduction in lesion diameter (2.1 mm) and consequent disease inhibition (84%) on the tomato fruit treated with methanol seed extracts. Scanning electron microscopy showed that extracts significantly damaged the morphology of hyphae and conidial structures. The FT-IR spectrum obtained from methanol extracts showed bands representing important bioactive compounds that possess antifungal activity. Based on our findings, fruit extracts can be further explored as a potential, excellent alternative approach to control the postharvest spots of tomato fruit.
本研究的目的是评估番茄果实提取物作为合成杀菌剂的替代品，用于对抗番茄果实黑斑病的病原菌——（弗里斯）凯斯勒菌。对番茄果肉和种子提取物的抗真菌活性进行了体内和体外测试。种子提取物在抑制该病菌方面比果肉提取物更有效。体外试验表明，甲醇种子提取物在最高浓度6%时对该病菌的径向菌丝生长具有最大抑制作用（90%）。同样，体内试验表明，用甲醇种子提取物处理的番茄果实病斑直径显著减小（2.1毫米），病害抑制率达84%。扫描电子显微镜显示，番茄提取物显著破坏了菌丝和分生孢子结构的形态。从甲醇提取物获得的傅里叶变换红外光谱显示出代表具有抗真菌活性的重要生物活性化合物的谱带。基于我们的研究结果，番茄果实提取物可作为一种潜在的、出色的替代方法，进一步用于控制番茄果实采后的黑斑病。
