Yan Shuai, Yue Yinzi, Zeng Li, Su Lianlin, Hao Min, Zhang Wei, Wang Xiaopeng
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China.
Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, China.
Front Chem. 2021 Apr 16;9:675346. doi: 10.3389/fchem.2021.675346. eCollection 2021.
Antioxidation is very important in medicine and food. The current evaluation technologies often have many shortcomings. In this work, an improved electrochemical sensing platform for the evaluation of antioxidant activity has been proposed. A hydrogel was prepared based on graphene oxide, zinc ions, and chitosan. Zinc ions play the role of crosslinking agents in hydrogels. The structure of chitosan can be destroyed by injecting hydrogen peroxide into the hydrogel, and the free zinc ions can diffuse to the surface of the electrode to participate in the electrochemical reaction. This electrochemical sensor can evaluate the antioxidant activity by comparing the current difference of zinc reduction before and after adding the antioxidant. With the help of graphene oxide, this hydrogel can greatly enhance the sensing effect. We conducted tests on 10 real samples. This proposed electrochemical platform has been successfully applied for evaluating the antioxidant activity of , and the results were compared to those obtained from the 2,2-diphenyl-1-picrylhydrazyl-based traditional analysis technique.
抗氧化作用在医学和食品领域非常重要。当前的评估技术往往存在许多缺点。在这项工作中，提出了一种用于评估抗氧化活性的改进型电化学传感平台。基于氧化石墨烯、锌离子和壳聚糖制备了一种水凝胶。锌离子在水凝胶中起交联剂的作用。通过向水凝胶中注入过氧化氢可破坏壳聚糖的结构，游离的锌离子可扩散到电极表面参与电化学反应。这种电化学传感器可通过比较添加抗氧化剂前后锌还原的电流差异来评估抗氧化活性。借助氧化石墨烯，这种水凝胶可大大增强传感效果。我们对10个实际样品进行了测试。所提出的电化学平台已成功应用于评估[具体物质]的抗氧化活性，并将结果与基于2,2-二苯基-1-苦基肼的传统分析技术所获得的结果进行了比较。
