Staicu Florentin-Daniel, Canha-Gouveia Analuce, Soriano-Úbeda Cristina, Martínez-Soto Juan Carlos, Adoamnei Evdochia, Chavarro Jorge E, Matás Carmen
Department of Physiology, Faculty of Veterinary Science, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), University of Murcia, Murcia, Spain.
Biomedical Research Institute of Murcia (IMIB), Murcia, Spain.
Front Cell Dev Biol. 2021 Apr 14;9:647002. doi: 10.3389/fcell.2021.647002. eCollection 2021.
Nitric oxide, a key regulatory molecule in the follicular fluid, has been suggested as a possible biomarker to predict ovarian response in stimulated cycles and the potential of the retrieved oocytes for developing high-quality embryos. Nevertheless, a consensus on whether or not nitric oxide can help in this context has not been reached. We simultaneously measured the oxidation products of nitric oxide, nitrite, and nitrate, via high-performance liquid chromatography (HPLC)-UV in follicular fluid samples from 72 oocyte donors. We found no associations of follicular fluid nitrite, nitrate, total nitric oxide, or nitrate/nitrite ratio with total or metaphase II (MII) oocyte yield. However, nitrite and nitrate levels were related to the yield of MII oocytes when this outcome was expressed as a proportion of all oocytes retrieved. The adjusted MII proportion in the lowest and highest nitrite levels were 68% (58-77%) and 79% (70-85%), respectively (p, linear trend = 0.02), whereas the adjusted MII proportion in extreme tertiles of nitrate levels were 79% (70-85%) and 68% (57-77%) (p, linear trend = 0.03). In addition, nitrate levels showed a suggestive inverse correlation with embryos with maximum or high potential of implantation ( = 0.07). These results suggest that the follicular fluid concentrations of nitrite and nitrate may be a useful tool in predicting how healthy oocyte donors respond to superovulation and the implantation potential of the embryos produced from their oocytes.
一氧化氮是卵泡液中的一种关键调节分子，已被认为是一种可能的生物标志物，用于预测刺激周期中的卵巢反应以及所获取卵母细胞发育高质量胚胎的潜力。然而，关于一氧化氮在这方面是否有帮助尚未达成共识。我们通过高效液相色谱（HPLC）-紫外法同时测量了来自72名卵母细胞捐赠者的卵泡液样本中一氧化氮的氧化产物亚硝酸盐和硝酸盐。我们发现卵泡液中亚硝酸盐、硝酸盐、总一氧化氮或硝酸盐/亚硝酸盐比值与总卵母细胞产量或中期II（MII）卵母细胞产量之间没有关联。然而，当将MII卵母细胞产量表示为所有获取卵母细胞的比例时，亚硝酸盐和硝酸盐水平与MII卵母细胞产量相关。亚硝酸盐水平最低和最高时调整后的MII比例分别为68%（58 - 77%）和79%（70 - 85%）（p，线性趋势 = 0.02），而硝酸盐水平处于极端三分位数时调整后的MII比例分别为79%（70 - 85%）和68%（57 - 77%）（p，线性趋势 = 0.03）。此外，硝酸盐水平与具有最大或高植入潜力的胚胎呈暗示性负相关（ = 0.07）。这些结果表明，卵泡液中亚硝酸盐和硝酸盐的浓度可能是预测健康卵母细胞捐赠者对超排卵的反应以及其卵母细胞所产生胚胎的植入潜力的有用工具。
