Dal Sasso Eleonora, Zamuner Annj, Filippi Andrea, Romanato Filippo, Palmosi Tiziana, Vedovelli Luca, Gregori Dario, Gómez Ribelles José Luís, Russo Teresa, Gloria Antonio, Iop Laura, Gerosa Gino, Dettin Monica
Department of Cardiac, Thoracic and Vascular Sciences and Venetian Institute of Molecular Medicine, Padua, Italy.
Department of Industrial Engineering, University of Padua, Padua, Italy.
Bioact Mater. 2021 Apr 12;6(11):3851-3864. doi: 10.1016/j.bioactmat.2021.04.003. eCollection 2021 Nov.
In the field of tissue regeneration, the lack of a stable endothelial lining may affect the hemocompatibility of both synthetic and biological replacements. These drawbacks might be prevented by specific biomaterial functionalization to induce selective endothelial cell (EC) adhesion. Decellularized bovine pericardia and porcine aortas were selectively functionalized with a REDV tetrapeptide at 10 M and 10 M working concentrations. The scaffold-bound peptide was quantified and REDV potential EC adhesion enhancement was evaluated by static seeding of human umbilical vein ECs. The viable cells and MTS production were statistically higher in functionalized tissues than in control. Scaffold histoarchitecture, geometrical features, and mechanical properties were unaffected by peptide anchoring. The selective immobilization of REDV was effective in accelerating ECs adhesion while promoting proliferation in functionalized decellularized tissues intended for blood-contacting applications.
在组织再生领域，缺乏稳定的内皮衬里可能会影响合成和生物替代物的血液相容性。通过特定的生物材料功能化以诱导选择性内皮细胞（EC）粘附，可以避免这些缺点。将去细胞牛心包和猪主动脉分别用工作浓度为10μM和10μM的REDV四肽进行选择性功能化。对支架结合的肽进行定量，并通过人脐静脉内皮细胞的静态接种评估REDV增强内皮细胞粘附的潜力。功能化组织中的活细胞和MTS产量在统计学上高于对照组。肽的锚定不影响支架的组织结构、几何特征和机械性能。REDV的选择性固定化可有效加速内皮细胞的粘附，同时促进用于血液接触应用的功能化去细胞组织中的细胞增殖。
