AIDS Institute and Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China.
STAR Protoc. 2021 Apr 14;2(2):100453. doi: 10.1016/j.xpro.2021.100453. eCollection 2021 Jun 18.
This protocol describes how to visualize surface protein-protein co-localization across a cell-cell interface between antigen-presenting γδ-T cells and CD4 T cells. By consolidating immunofluorescence assay, confocal microscopy and 3D imaging analysis, it enables assessment of interaction between cell surface proteins such as Δ42PD1 and TLR4 between co-cultured γδ-T and CD4 T cells. This protocol can be applied to study a surface protein of interest and its potential interaction with a target cell/protein at the cell-cell interface. For complete details on the use and execution of this profile, please refer to Mo et al. (2020).
本方案描述了如何在抗原呈递 γδ-T 细胞和 CD4 T 细胞之间的细胞-细胞界面上可视化表面蛋白-蛋白的共定位。通过整合免疫荧光检测、共聚焦显微镜和 3D 成像分析，能够评估共培养的 γδ-T 和 CD4 T 细胞之间的细胞表面蛋白（如 Δ42PD1 和 TLR4）之间的相互作用。本方案可用于研究表面蛋白及其与细胞-细胞界面上靶细胞/蛋白的潜在相互作用。有关该方案的详细使用和执行信息，请参见 Mo 等人（2020 年）。
