Moacă Elena-Alina, Watz Claudia-Geanina, Socoliuc Vlad, Racoviceanu Roxana, Păcurariu Cornelia, Ianoş Robert, Cîntă-Pînzaru Simona, Tudoran Lucian Barbu, Nekvapil Fran, Iurciuc Stela, Șoica Codruța, Dehelean Cristina-Adriana
Faculty of Pharmacy, Victor Babeș University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania.
Research Centre for Pharmaco-Toxicological Evaluation, "Victor Babeș" University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, RO-300041 Timișoara, Romania.
Nanomaterials (Basel). 2021 Apr 30;11(5):1189. doi: 10.3390/nano11051189.
Magnetic iron oxide nanoparticles are the most desired nanomaterials for biomedical applications due to their unique physiochemical properties. A facile single-step process for the preparation of a highly stable and biocompatible magnetic colloidal suspension based on citric-acid-coated magnetic iron oxide nanoparticles used as an effective heating source for the hyperthermia treatment of cancer cells is presented. The physicochemical analysis revealed that the magnetic colloidal suspension had a z-average diameter of 72.7 nm at 25 °C with a polydispersity index of 0.179 and a zeta potential of -45.0 mV, superparamagnetic features, and a heating capacity that was quantified by an intrinsic loss power analysis. Raman spectroscopy showed the presence of magnetite and confirmed the presence of citric acid on the surfaces of the magnetic iron oxide nanoparticles. The biological results showed that breast adenocarcinoma cells (MDA-MB-231) were significantly affected after exposure to the magnetic colloidal suspension with a concentration of 30 µg/mL 24 h post-treatment under hyperthermic conditions, while the nontumorigenic (MCF-10A) cells exhibited a viability above 90% under the same thermal setup. Thus, the biological data obtained in the present study clearly endorse the need for further investigations to establish the clinical biological potential of synthesized magnetic colloidal suspension for magnetically triggered hyperthermia.
磁性氧化铁纳米颗粒因其独特的物理化学性质而成为生物医学应用中最理想的纳米材料。本文介绍了一种简便的单步制备方法，该方法基于柠檬酸包覆的磁性氧化铁纳米颗粒制备出高度稳定且具有生物相容性的磁性胶体悬浮液，该悬浮液可用作癌细胞热疗的有效热源。物理化学分析表明，该磁性胶体悬浮液在25℃时的z平均直径为72.7nm，多分散指数为0.179，zeta电位为-45.0mV，具有超顺磁性特征，并且通过固有损耗功率分析对其加热能力进行了量化。拉曼光谱显示存在磁铁矿，并证实磁性氧化铁纳米颗粒表面存在柠檬酸。生物学结果表明，在热疗条件下，暴露于浓度为30μg/mL的磁性胶体悬浮液24小时后，乳腺腺癌细胞（MDA-MB-231）受到显著影响，而非致瘤性（MCF-10A）细胞在相同热设置下的存活率高于90%。因此，本研究获得的生物学数据明确支持需要进一步研究，以确定合成的磁性胶体悬浮液在磁触发热疗中的临床生物学潜力。
