Department of Critical Care Medicine, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, China.
Department of Pathophysiology, Zhejiang University Medical College, Hangzhou, China.
World Neurosurg. 2022 Sep;165:e1-e11. doi: 10.1016/j.wneu.2021.04.114. Epub 2021 May 3.
Mesenchymal stem cells (MSCs) have been shown to enhance neurological recovery after stroke. A rat middle cerebral artery occlusion model was designed to assess neuroprotective effects of stroke pretreated MSCs on cerebral ischemia/reperfusion injury.
MSCs were isolated and cultured in medium with 10% fetal bovine serum, normal control serum, or stroke serum (SS). MSCs were then injected into rats (n = 6 in each group) 1 day after middle cerebral artery occlusion, and feeding continued for 28 days. A battery of behavioral tests, 2,3,5-triphenyltetrazolium chloride staining, hematoxylin-eosin staining, enzyme-linked immunosorbent assay, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay were used to assess neural injury. To detect enhancement of neuronal regeneration and angiogenesis, immunofluorescence and Western blotting were performed to assess expression of trophic factors and growth factors.
After treatment, behavior of rats improved significantly. Infarction area, brain lesion, and apoptosis cells were significantly decreased in the SS-MSCs group compared with the other groups. SS-MSCs also modulated inflammation by attenuating inflammatory cytokines. Furthermore, the number of neurogenesis-positive cells and expression of trophic factors and growth factors were significantly higher in the SS-MSCs group compared with the others. MSCs cultured with fetal bovine serum and normal control serum showed differences in expression of trophic factors and growth factors, but the results were not as good as with SS-MSCs.
Administration of SS-MCSs after reperfusion led to neuroprotection by inducing the recovery process, including improving pathological changes, behavioral improvement, neurogenesis, suppression of apoptosis and inflammation, and angiogenesis.
间充质干细胞（MSCs）已被证明可增强中风后的神经恢复。设计了大鼠大脑中动脉闭塞模型，以评估预处理 MSC 对脑缺血/再灌注损伤的神经保护作用。
将 MSCs 分离并在含有 10%胎牛血清、正常对照血清或中风血清（SS）的培养基中培养。然后将 MSCs 在大脑中动脉闭塞后 1 天注射到大鼠（每组 6 只）中，并继续喂养 28 天。一系列行为测试、2,3,5-三苯基氯化四氮唑染色、苏木精-伊红染色、酶联免疫吸附测定和末端脱氧核苷酸转移酶 dUTP 缺口末端标记测定用于评估神经损伤。为了检测神经元再生和血管生成的增强，进行免疫荧光和 Western blot 分析以评估营养因子和生长因子的表达。
治疗后，大鼠的行为明显改善。与其他组相比，SS-MSCs 组的梗塞面积、脑损伤和凋亡细胞明显减少。SS-MSCs 还通过减弱炎症细胞因子来调节炎症。此外，SS-MSCs 组的神经发生阳性细胞数量和营养因子和生长因子的表达明显高于其他组。用胎牛血清和正常对照血清培养的 MSCs 在营养因子和生长因子的表达上存在差异，但结果不如 SS-MSCs 好。
再灌注后给予 SS-MCSs 可通过诱导恢复过程来实现神经保护，包括改善病理变化、行为改善、神经发生、抑制凋亡和炎症以及血管生成。
