仿生海绵可改善容积性肌肉损失后的肌肉结构和功能。
Biomimetic sponges improve muscle structure and function following volumetric muscle loss.
机构信息
Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, St. Louis, Missouri, USA.
School of Biological Sciences, College of Natural and Health Sciences, University of Northern Colorado, Greeley, Colorado, USA.
出版信息
J Biomed Mater Res A. 2021 Nov;109(11):2280-2293. doi: 10.1002/jbm.a.37212. Epub 2021 May 7.
Abstract
Skeletal muscle is inept in regenerating after traumatic injuries such as volumetric muscle loss (VML) due to significant loss of various cellular and acellular components. Currently, there are no approved therapies for the treatment of muscle tissue following trauma. In this study, biomimetic sponges composed of gelatin, collagen, laminin-111, and FK-506 were used for the treatment of VML in a rodent model. We observed that biomimetic sponge treatment improved muscle structure and function while modulating inflammation and limiting the extent of fibrotic tissue deposition. Specifically, sponge treatment increased the total number of myofibers, type 2B fiber cross-sectional area, myosin: collagen ratio, myofibers with central nuclei, and peak isometric torque compared to untreated VML injured muscles. As an acellular scaffold, biomimetic sponges may provide a promising clinical therapy for VML.
摘要
骨骼肌在创伤后再生能力较差，例如由于各种细胞和无细胞成分的大量丢失而导致的容积性肌肉损失（VML）。目前，还没有批准用于治疗创伤后肌肉组织的疗法。在这项研究中，使用由明胶、胶原蛋白、层粘连蛋白-111 和 FK-506 组成的仿生海绵来治疗啮齿动物模型中的 VML。我们观察到仿生海绵治疗改善了肌肉结构和功能，同时调节了炎症并限制了纤维组织沉积的程度。具体来说，与未治疗的 VML 损伤肌肉相比，海绵治疗增加了肌纤维总数、2B 型纤维横截面积、肌球蛋白：胶原蛋白比、具有中央核的肌纤维和峰值等长扭矩。作为无细胞支架，仿生海绵可能为 VML 提供一种有前途的临床治疗方法。
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