College of Life Science, Yantai University, No.30, Qing Quan Road, Yantai, Shandong Province, 264005, PR China.
College of Life Science, Yantai University, No.30, Qing Quan Road, Yantai, Shandong Province, 264005, PR China.
J Tissue Viability. 2021 Aug;30(3):454-461. doi: 10.1016/j.jtv.2021.04.008. Epub 2021 Apr 30.
The aim of the current study was to develop collagen-based bi-layered composite dressings with antibacterial property and evaluate the efficiency for wound healing.
A bi-layered composite wound dressing was fabricated using two marine biomacromolecules (collagen and chitosan or carboxymethyl chitosan). Non-crosslinked and N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/N-Hydroxy succinimide (EDC/NHS) cross-linked collagen sponges fabricated by vacuum freeze-drying technology was used as the inner layer. The medical spun-laced nonwoven coated with chitosan and carboxymethyl chitosan was used as the outer layer. The antibacterial activities against E. coli and S. aureus were evaluated by the inhibition zone assay. Deep second-degree scald model was performed to evaluate the efficiency of bi-layered composite dressings for wound healing.
In view of comprehensive evaluation of appearance and in vitro antibacterial activity, medical spun-laced nonwoven coated with 3% of chitosan solution was chosen to be used as the optimized preparation conditions to produce the outer layer of composite dressing, which acted as a barrier against microorganisms and provided mechanical support. Furthermore, the results of wound closure and histopathological analysis indicated that EDC/NHS cross-linked collagen-based bi-layered composite dressing was superior to non-crosslinked and commercial products, which stimulated the wound healing process and accomplished deep second-degree scalded skin healing within a time span of 28 days.
The EDC/NHS cross-linked collagen-based bi-layered composite dressing had immense potential to be applied for an ideal wound dressing for more efficient and faster wound healing. Therefore, the findings provided the essential theoretical basis for great potential of collagen-based composite dressing used in wound healing applications.
本研究旨在开发具有抗菌性能的胶原基双层复合敷料，并评估其在伤口愈合中的效率。
采用两种海洋生物大分子（胶原和壳聚糖或羧甲基壳聚糖）制备双层复合伤口敷料。使用真空冷冻干燥技术制备的非交联和 N-乙基-N'-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）交联胶原海绵作为内层。将涂有壳聚糖和羧甲基壳聚糖的医用纺粘非织造布用作外层。通过抑菌圈试验评估对大肠杆菌和金黄色葡萄球菌的抗菌活性。通过深二度烫伤模型评估双层复合敷料促进伤口愈合的效率。
鉴于外观和体外抗菌活性的综合评价，选择涂有 3%壳聚糖溶液的医用纺粘非织造布作为优化的制备条件，用于生产复合敷料的外层，作为微生物的屏障并提供机械支撑。此外，伤口闭合和组织病理学分析的结果表明，EDC/NHS 交联的胶原基双层复合敷料优于非交联和商业产品，可刺激伤口愈合过程，并在 28 天内完成深二度烫伤皮肤的愈合。
EDC/NHS 交联的胶原基双层复合敷料具有巨大的潜力，可作为理想的伤口敷料，实现更高效、更快的伤口愈合。因此，这些发现为胶原基复合材料在伤口愈合应用中的巨大潜力提供了必要的理论基础。
