Department of Molecular Biology, Princeton University, Princeton, New Jersey.
Department of Molecular Biology, Princeton University, Princeton, New Jersey.
Biophys J. 2021 Oct 5;120(19):4149-4161. doi: 10.1016/j.bpj.2021.04.035. Epub 2021 May 6.
The last decade has seen a major expansion in development of live biosensors, the tools needed to genetically encode them into model organisms, and the microscopic techniques used to visualize them. When combined, these offer us powerful tools with which to make fundamental discoveries about complex biological processes. In this review, we summarize the availability of biosensors to visualize an essential cellular process, the cell cycle, and the techniques for single-cell tracking and quantification of these reporters. We also highlight studies investigating the connection of cellular behavior to the cell cycle, particularly through live imaging, and anticipate exciting discoveries with the combination of these technologies in developmental contexts.
过去十年中，活体生物传感器的发展、将其遗传编码到模式生物中的工具以及用于对其进行可视化的显微镜技术都取得了重大进展。这些技术的结合为我们提供了强大的工具，使我们能够对复杂的生物过程进行基础性的发现。在这篇综述中，我们总结了可用于可视化细胞周期这一基本细胞过程的生物传感器，以及用于单细胞跟踪和这些报告基因定量的技术。我们还强调了通过活体成像研究细胞行为与细胞周期之间联系的研究，并期待在发育背景下结合这些技术会带来令人兴奋的发现。
