Gallagher M J, Chan Y L, Lin A, Wool I G
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
DNA. 1988 May;7(4):269-73. doi: 10.1089/dna.1988.7.269.
The amino acid sequence of rat ribosomal protein L36a, which may form part of the peptidyl transferase center, was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the amino-terminal amino acid sequence of the protein. Ribosomal protein L36a contains 105 amino acids (the amino-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 12,311. Hybridization of the cDNA to digests of nuclear DNA suggests that there are multiple copies of the L36a gene. Rat ribosomal protein L36a is homologous to a protein HL44 present in ribosomes of humans and protein 44 from Saccharomyces cerevisiae ribosomes.
大鼠核糖体蛋白L36a的氨基酸序列可能是肽基转移酶中心的一部分，它是从重组cDNA中的核苷酸序列推导出来的，并通过该蛋白的氨基末端氨基酸序列得到证实。核糖体蛋白L36a含有105个氨基酸（mRNA翻译后氨基末端的甲硫氨酸被去除），分子量为12311。cDNA与核DNA消化物的杂交表明L36a基因有多个拷贝。大鼠核糖体蛋白L36a与人类核糖体中存在的一种蛋白HL44以及酿酒酵母核糖体中的蛋白44同源。
