Wengert Peter C, Wong Narayan H, Barton Hazel A, Gan Han Ming, Hudson André O, Savka Michael A
Thomas H. Gosnell School of Life Sciences, College of Science, Rochester Institute of Technology, Rochester, NY, 14623, USA.
Department of Biology, University of Akron, Akron, OH, USA.
BMC Res Notes. 2021 May 8;14(1):175. doi: 10.1186/s13104-021-05589-6.
To characterize the bacterial community of Wind Cave's Madison aquifer through whole-genome sequencing, and to better understand the bacterial ecology by identifying genes involved in acyl-homoserine lactone (AHL) based quorum-sensing (QS) systems.
Genome-based taxonomic classification revealed the microbial richness present in the pristine Madison aquifer. The strains were found to span eleven genera and fourteen species, of which eight had uncertain taxonomic classifications. The genomes of strains SD129 and SD340 were found to contain the archetypical AHL QS system composed of two genes, luxI and luxR. Surprisingly, the genomes of strains SD115, SD129, SD274 and SD316 were found to contain one to three luxR orphans (solos). Strain SD129, besides possessing an archetypical AHL QS luxI-luxR pair, also contained two luxR solos, while strain SD316 contained three LuxR solos and no luxI-luxR pairs. The ligand-binding domain of two LuxR solos, one each from strains SD129 and SD316, were found to contain novel substitutions not previously reported, thus may represent two LuxR orphans that detection and response to unknown self-produced signal(s), or to signal(s) produced by other organisms.
通过全基因组测序对风洞麦迪逊含水层的细菌群落进行特征分析，并通过鉴定参与基于酰基高丝氨酸内酯（AHL）的群体感应（QS）系统的基因，更好地了解细菌生态学。
基于基因组的分类学分类揭示了原始麦迪逊含水层中存在的微生物丰富度。发现这些菌株涵盖11个属和14个种，其中8个的分类学分类不确定。发现菌株SD129和SD340的基因组包含由luxI和luxR两个基因组成的典型AHL QS系统。令人惊讶的是，发现菌株SD115、SD129、SD274和SD316的基因组包含1至3个luxR孤儿（单独基因）。菌株SD129除了拥有典型的AHL QS luxI-luxR对之外，还包含两个luxR单独基因，而菌株SD316包含三个LuxR单独基因且没有luxI-luxR对。发现来自菌株SD129和SD316的两个LuxR单独基因的配体结合结构域含有以前未报道的新替换，因此可能代表两个对未知自身产生的信号或其他生物体产生的信号进行检测和响应的LuxR孤儿。
