Kumar Heerman Kumar Sandra, Gan Han Ming, Tan Mun Hua, Eng Wilhelm Wei Han, Barton Hazel A, Hudson André O, Savka Michael A
School of Science, Monash University Malaysia, Bandar Sunway, Selangor, Malaysia.; Genomics Facility, Tropical Medicine and Biology Platform, Monash University Malaysia, Bandar Sunway, Selangor, Malaysia.
Department of Biology, University of Akron, Akron, Ohio, USA.
J Genomics. 2017 Jan 18;5:12-15. doi: 10.7150/jgen.17863. eCollection 2017.
A total of eight strains were isolated from two pristine cave environments. One strain was isolated from a cave water pool located in the Wind Cave National Park, South Dakota, USA and the remaining seven strains were isolated from Lechuguilla Cave of Carlsbad Caverns National Park, New Mexico, USA. Whole genome sequencing and comparative genomic analyses of the eight isolates compared to various type strains from the genera and demonstrates that although members in these genera can be phylogenetically separated into two distinct clades, the percentage of conserved proteins (POCP) between various type strains from and are consistently higher than 50%, providing strong genomic evidence to support the classification of the genera and into a single genus.
总共从两个原始洞穴环境中分离出了8株菌株。其中1株从美国南达科他州风洞国家公园的一个洞穴水池中分离得到，其余7株从美国新墨西哥州卡尔斯巴德洞穴国家公园的龙舌兰洞穴中分离得到。对这8株分离株进行全基因组测序，并与来自相关属的各种模式菌株进行比较基因组分析，结果表明，尽管这些属中的成员在系统发育上可分为两个不同的进化枝，但相关属的各种模式菌株之间的保守蛋白百分比（POCP）始终高于50%，这为支持将相关属归为一个单属提供了有力的基因组证据。
