Zhu Shouhong, Wang Xinyu, Chen Wei, Yao Jinbo, Li Yan, Fang Shengtao, Lv Youjun, Li Xiaxuan, Pan Jingwen, Liu Chunyan, Li Qiulin, Zhang Yongshan
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China.
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China.
Int J Biol Macromol. 2021 Jul 31;183:1257-1269. doi: 10.1016/j.ijbiomac.2021.05.023. Epub 2021 May 18.
Members of DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (DMP) gene family, a type of plant-specific membrane proteins, have been proposed to function in various physiological processes such as reproductive development and senescence in plants. Here, a total of 174 DMP genes were identified and analyzed in 16 plant species (including 58 DMPs in four cotton species). Phylogenetic analysis showed that these DMPs could be clustered into five subfamilies (I-V). 137 duplicated cotton gene pairs were identified and most duplicate events were formed by whole-genome duplication (WGD)/segmental duplications. Expression analysis revealed that most of cotton DMPs were mainly expressed in the reproductive organs (the sepal, petal, pistil and anther) and the fiber of secondary cell wall stage. GhDMPs promoter regions containing the different cis-elements also showed different responses to abiotic stress. In addition, gene interaction networks showed that DMPs, as an endomembrane system, were involved in plant senescence process and flower reproductive development. We speculated GhDMP8-A/-D, GbDMP8-A/-D could be used as some candidate gene for inducing cotton haploid. This genome-wide study provides a systematic analysis of the cotton DMP gene family, and further insights towards understanding the potential functions of candidate genes.
未知功能域679膜蛋白（DMP）基因家族的成员是一类植物特有的膜蛋白，据推测其在植物的各种生理过程中发挥作用，如生殖发育和衰老。在此，我们在16种植物物种中总共鉴定并分析了174个DMP基因（包括四个棉花物种中的58个DMP）。系统发育分析表明，这些DMP可聚类为五个亚家族（I-V）。鉴定出137对重复的棉花基因对，且大多数重复事件是由全基因组复制（WGD）/片段重复形成的。表达分析显示，大多数棉花DMP主要在生殖器官（萼片、花瓣、雌蕊和花药）以及次生细胞壁阶段的纤维中表达。含有不同顺式元件的GhDMPs启动子区域对非生物胁迫也表现出不同的响应。此外，基因相互作用网络表明，DMP作为内膜系统，参与了植物衰老过程和花的生殖发育。我们推测GhDMP8-A/-D、GbDMP8-A/-D可作为诱导棉花单倍体的一些候选基因。这项全基因组研究为棉花DMP基因家族提供了系统分析，并为理解候选基因的潜在功能提供了进一步的见解。
