Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, China.
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138, United States.
ACS Biomater Sci Eng. 2021 May 10;7(5):1827-1835. doi: 10.1021/acsbiomaterials.0c01271. Epub 2020 Oct 1.
The progressively increasing degree of multiple antibiotic resistance in Gram-negative bacteria challenges the treatment of severe bacterial infection-induced chronic skin wounds. To address this problem, we developed a straightforward strategy to prepare a composite film consisting of antibacterial nanoparticles (4,6-diamino-2-pyrimidinethiol-functionalized gold nanoparticles, DAPT-Au NPs) and a silk fibroin (SF) mixed-matrix membrane (DAPT-Au-SF MMM) as a wound dressing for treating multidrug-resistant (MDR) induced infection. The good hydrophilicity of SF allows the highly effective release of DAPT-Au NPs from the composite film to combat pathogens within minutes. The antibacterial activity of the composite film is maintained regardless of antimicrobial susceptibility. DAPT-Au-SF MMMs also promote healing in rat wounds infected by clinically isolated MDR . Our findings provide a new strategy to extend the use of gold nanomaterials and SF-based wound dressings, especially against drug-resistant bacterial infections.
革兰氏阴性菌的多重抗生素耐药性逐渐增加，给严重细菌感染引起的慢性皮肤伤口的治疗带来了挑战。为了解决这个问题，我们开发了一种简单的策略，制备了一种由抗菌纳米粒子（4,6-二氨基-2-嘧啶硫醇功能化的金纳米粒子，DAPT-Au NPs）和丝素蛋白（SF）混合基质膜（DAPT-Au-SF MMM）组成的复合膜，作为治疗多药耐药（MDR）诱导感染的伤口敷料。SF 的良好亲水性允许 DAPT-Au NPs 从复合膜中高效释放，在数分钟内对抗病原体。复合膜的抗菌活性不受抗菌药物敏感性的影响。DAPT-Au-SF MMM 还促进了由临床分离的 MDR 感染的大鼠伤口的愈合。我们的研究结果为延长金纳米材料和基于 SF 的伤口敷料的使用提供了一种新策略，特别是针对耐药性细菌感染。
