Bohall Peter B, Bell Stephen D
Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, United States.
Department of Biology, Indiana University, Bloomington, IN, United States.
Front Microbiol. 2021 Apr 22;12:666974. doi: 10.3389/fmicb.2021.666974. eCollection 2021.
Across the three domains of life, B-family DNA polymerases play a variety of roles in both DNA repair and DNA replication processes. We examine the phenotypic consequences of loss of the putative repair polymerases PolB2 and/or PolB3 in the crenarchaeon . We detect a modest growth advantage when cells lacking the polymerase are grown in unperturbed conditions. Further, we observe a striking insensitivity of the mutant lines to acute treatment with the oxidizing agent, hydrogen peroxide. In addition, cells lacking PolB3 show enhanced sensitivity to the DNA damaging agent 4-NQO. Our data therefore suggest that these non-essential DNA polymerases may influence DNA repair pathway choice in these hyperthermophilic aerobes.
在生命的三个领域中，B族DNA聚合酶在DNA修复和DNA复制过程中发挥着多种作用。我们研究了泉古菌中假定的修复聚合酶PolB2和/或PolB3缺失的表型后果。当缺乏聚合酶的细胞在未受干扰的条件下生长时，我们检测到适度的生长优势。此外，我们观察到突变株系对氧化剂过氧化氢的急性处理具有显著的不敏感性。此外，缺乏PolB3的细胞对DNA损伤剂4-硝基喹啉氧化物表现出增强的敏感性。因此，我们的数据表明，这些非必需的DNA聚合酶可能会影响这些嗜热需氧菌中的DNA修复途径选择。
