Abu Farahziela, Mat Taib Che Norma, Mohd Moklas Mohamad Aris, Mohd Akhir Sobri
Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor (Darul Ehsan), Malaysia.
Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor (Darul Ehsan), Malaysia.
Evid Based Complement Alternat Med. 2017;2017:2907219. doi: 10.1155/2017/2907219. Epub 2017 Jul 6.
Antioxidant properties of crude extract, partition extract, and fermented medium from (DS) flower were investigated. The oven-dried DS flower was extracted using 100% methanol (w/v), 100% ethanol (w/v), and 100% water (w/v). The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract) and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on DS flower medium showed a potential in increasing the phenolic content and DPPH scavenging activity. The TPC of final fermented medium showed approximately 18% increment, while the DPPH of fermented medium increased significantly to approximately 80% at the end of the fermentation. (DS) flower showed very good potential properties of antioxidant in crude extract and partition extract as well as better antioxidant activity in the flower fermented medium.
对(DS)花的粗提物、分离提取物和发酵培养基的抗氧化特性进行了研究。将烘干的DS花分别用100%甲醇(w/v)、100%乙醇(w/v)和100%水(w/v)进行提取。100%甲醇粗提物的总酚含量最高(40.33±mg GAE/g提取物),并且在DPPH、ABTS和FRAP测定中表现出最佳的抗氧化性能。抗氧化活性与总酚含量之间的相关关系表明,酚类化合物是该花提取物中的主要抗氧化成分。DS花培养基上的微生物发酵显示出增加酚含量和DPPH清除活性的潜力。最终发酵培养基的总酚含量增加了约18%,而发酵培养基的DPPH在发酵结束时显著增加至约80%。(DS)花在粗提物和分离提取物中显示出非常好的抗氧化潜在特性,并且在花发酵培养基中具有更好的抗氧化活性。