Bioassay-Guided Fractionation Networking for Discovery of Biofungicides from Cultivated .

Considering the detrimental impacts of the current pesticides on the biotic components of the biosphere, the development of novel pesticides is vital. Plant-derived biopesticides have emerged as popular alternatives to create a safer and more sustainable agriculture model. This study aims to validate the previous bioguided fractionation of endemic Canary Islands sage, , as a potential source of botanical pesticides using a cultivation process. Accordingly, the bioassay-guided fractionation of the ethanolic extract of the leaves of cultivated on the phytopathogenic fungal mycelia of , , and yielded six known terpenoids. Their abietane diterpenoid-type (-) and sesquiterpenoid () structures were established based on spectroscopic and spectrometric analysis. This strategy identified one abietane diterpenoid, salviol (), as a potential candidate for the future development of biofungicides with similar potency towards the assayed phytopathogenic fungi to commercial fungicides. Salviol worked in a concentration-dependent manner. Overall, this study reinforces the potential of abietane-type diterpenoids as promising agrochemical lead compounds against infectious diseases caused by phytopathogenic fungi and validates the cultivation of as a potential source of plant-derived biopesticides.