Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou, Hainan, 570228, People's Republic of China.
College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya, Hainan, 572022, People's Republic of China.
BMC Genomics. 2020 Apr 3;21(1):275. doi: 10.1186/s12864-020-6695-9.
Phytopathogens secreted effectors during host colonization to suppress or trigger plant immunity. Identification of new effectors is one of the research focuses in recent years. There is only a limited knowledge about effectors of Fusarium oxysporum f. sp. Cubense tropical race 4 (Foc TR4), the causal agent of wilt disease in Cavendish banana.
Two transcription factors, SGE1 and FTF1, were constitutively over-expressed in Foc TR4 to partially mimic the in-planta state. Secreted proteins with high purity were prepared through a two-round extraction method. Then the secretome were analyzed via label free proteomics method. A total of 919 non-redundant proteins were detected, of which 74 proteins were predicted to be effector candidates. Among these candidates, 29 were up-regulated and 13 down-regulated in the strain over-expressing SGE1 and FTF1, 8 were up-regulated and 4 down-regulated in either SGE1 or FTF1 over expression strain.
Through label free proteomics analysis, a series of effector candidates were identified in secretome of Foc TR4. Our work put a foundation for functional research of these effectors.
植物病原菌在宿主定殖过程中会分泌效应子来抑制或触发植物免疫。鉴定新的效应子是近年来的研究重点之一。关于尖孢镰刀菌古巴专化型热带 4 号（Foc TR4）的效应子（引起卡文迪什香蕉枯萎病的病原体），我们的了解非常有限。
在 Foc TR4 中组成型过表达两个转录因子 SGE1 和 FTF1，部分模拟了体内状态。通过两轮提取法制备了高纯度的分泌蛋白。然后通过无标记蛋白质组学方法分析了分泌组。共检测到 919 个非冗余蛋白，其中 74 个被预测为效应子候选物。在过表达 SGE1 和 FTF1 的菌株中，有 29 个上调，13 个下调，在 SGE1 或 FTF1 过表达菌株中，有 8 个上调，4 个下调。
通过无标记蛋白质组学分析，在 Foc TR4 的分泌组中鉴定出了一系列效应子候选物。我们的工作为这些效应子的功能研究奠定了基础。
