Girjes A A, Hugall A F, Timms P, Lavin M F
Department of Biochemistry, University of Queensland, Brisbane, Australia.
Infect Immun. 1988 Aug;56(8):1897-900. doi: 10.1128/iai.56.8.1897-1900.1988.
While several diseases associated with Chlamydia psittaci infection have been reported in Phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. In this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strains of C. psittaci infect koalas; one strain was isolated from the conjunctivae, and the other was isolated from the urogenital tract and the rectum. A gene probe, pFEN207, containing the coding sequence for an enzyme involved in the biosynthesis of the chlamydial genus-specific lipopolysaccharide antigen, and a separate probe, pCPML-4N, prepared from a DNA fragment of a koala-infecting strain of C. psittaci, were used to determine the patterns of hybridization in the koala-infecting strains; these patterns were found to be quite distinct from those observed with C. psittaci isolates from other animals. We also demonstrated by hybridization analysis with an avian strain plasmid that all three koala urogenital isolates contain a plasmid and that there is no evidence for the presence of a homologous plasmid in any of the ocular isolates.
虽然在树袋熊（考拉）中已报告了几种与鹦鹉热衣原体感染相关的疾病，但尚不清楚是一种还是多种衣原体菌株所致。在本研究中，我们通过限制性酶切和基因探针分析提供了证据，表明两种截然不同的鹦鹉热衣原体菌株感染考拉；一种菌株从结膜中分离得到，另一种从泌尿生殖道和直肠中分离得到。使用一个含有参与衣原体属特异性脂多糖抗原生物合成的一种酶编码序列的基因探针pFEN207，以及一个从感染考拉的鹦鹉热衣原体菌株的DNA片段制备的单独探针pCPML - 4N，来确定感染考拉菌株中的杂交模式；发现这些模式与从其他动物分离的鹦鹉热衣原体菌株所观察到的模式截然不同。我们还通过与一种禽源菌株质粒的杂交分析证明，所有三个考拉泌尿生殖道分离株都含有一个质粒，并且在任何眼部分离株中均没有同源质粒存在的证据。
