J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL USA.
Preston A. Wells, Jr. Center for Brain Tumor Therapy, University of Florida, Gainesville, FL USA.
Nanotheranostics. 2021 Apr 27;5(4):431-444. doi: 10.7150/ntno.55165. eCollection 2021.
Adoptive cellular therapy (ACT) is a potent strategy to boost the immune response against cancer. ACT is effective against blood cancers but faces challenges in treating solid tumors. A critical step for the success of ACT immunotherapy is to achieve efficient trafficking and persistence of T cells to solid tumors. Non-invasive tracking of the accumulation of adoptively transferred T cells to tumors would greatly accelerate development of more effective ACT strategies. We demonstrate the use of magnetic particle imaging (MPI) to non-invasively track ACT T cells in a mouse model of brain cancer. Magnetic labeling did not impair primary tumor-specific T cells and MPI allowed the detection of labeled T cells in the brain after intravenous or intracerebroventricular administration. These results support the use of MPI to track adoptively transferred T cells and accelerate the development of ACT treatments for brain tumors and other cancers.
过继性细胞疗法（ACT）是增强机体针对癌症免疫反应的有效策略。ACT 对血液癌症具有疗效，但在治疗实体瘤方面面临挑战。ACT 免疫疗法成功的一个关键步骤是实现 T 细胞向实体瘤的有效转运和持久性。对过继转移 T 细胞在肿瘤中积累的非侵入性跟踪，将极大地加速更有效的 ACT 策略的发展。我们证明了使用磁粒子成像（MPI）在脑癌小鼠模型中对 ACT T 细胞进行非侵入性跟踪。磁标记不会损害原发性肿瘤特异性 T 细胞，并且 MPI 允许在静脉内或脑室内给药后检测到大脑中的标记 T 细胞。这些结果支持使用 MPI 来跟踪过继转移的 T 细胞，并加速 ACT 治疗脑肿瘤和其他癌症的发展。
