Biomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, São Paulo, Brazil.
Department of Structural and Functional Biology, Institute of Biology, University of Campinas-UNICAMP, São Paulo, Brazil.
Microsc Res Tech. 2021 Nov;84(11):2588-2597. doi: 10.1002/jemt.23809. Epub 2021 May 11.
Acmella oleracea contains spilanthol as the main active compound, which possesses analgesic and anti-inflammatory effects that can favor tendon reorganization. To analyze the effect of A. oleracea on the content and organization of collagen in injured tendons, the calcaneal tendon of male Lewis rats was partially transected and treated at the site of injury with a topical application of 20% A. oleracea ointment (AO group) or with the ointment base without the plant extract (B group). The animals were euthanized 21 days after partial transection. Higher collagen concentration was observed in the AO group than in the B group, and morphological analysis using polarization microscopy showed higher birefringence in the AO group than in the B group, indicating higher collagen organization. No difference was observed in the number of fibroblasts, blood vessels, proteoglycan distribution, and maximum load between the B and AO groups. In conclusion, topical application of 20% A. oleracea ointment increased the molecular organization and content of collagen, thus indicating a potential application in tendon repair. Studies on the later phases of the tendon healing process are necessary to demonstrate the possible biomechanical changes after the application of A. oleracea ointment.
辣木叶含有醉椒素作为主要活性化合物，具有镇痛和抗炎作用，有利于肌腱的重新组织。为了分析辣木叶对损伤肌腱中胶原含量和组织的影响，将雄性 Lewis 大鼠跟腱部分横断，并在损伤部位局部应用 20%辣木叶软膏（AO 组）或不含植物提取物的软膏基质（B 组）进行处理。部分横断后 21 天处死动物。AO 组的胶原浓度高于 B 组，偏光显微镜下的形态分析显示 AO 组的双折射高于 B 组，表明胶原组织更高。B 组和 AO 组之间的成纤维细胞数量、血管数量、蛋白聚糖分布和最大载荷没有差异。总之，局部应用 20%辣木叶软膏增加了胶原的分子组织和含量，因此表明其在肌腱修复中有潜在的应用。有必要对肌腱愈合过程的后期阶段进行研究，以证明应用辣木叶软膏后的可能生物力学变化。
