Siddiqi Muhammad Zubair, Sambath Ponnareay, Im Wan-Taek
AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Jungang-ro Anseong-si, Gyeonggi-do 17579, Republic of Korea.
Department of Biotechnology, Hankyong National University, 327 Jungang-ro Anseong-si, Gyeonggi-do 17579, Republic of Korea.
Int J Syst Evol Microbiol. 2021 May;71(5). doi: 10.1099/ijsem.0.004793.
The conversion of major ginsenosides into minor ginsenosides attracts a lot of interest because of their biological and pharmaceutical activities. Therefore, for the conversion of ginsenosides, finding a novel competent glycoside hydrolase-producing bacterial strain is useful for future research studies and the mass production of minor ginsenosides. Wastewater samples were collected and screened for novel glycoside hydrolase bacterial strains using Reasoner's 2A+aesculin agar medium. As a result, a novel glycoside hydrolase positive bacterial strain (SB-02) was identified and subjected to a polyphasic taxonomic analysis. Based on genome analysis, strain SB-02 was found to be affiliated with the family and have less than 92.8 % sequence similarity to other members of the same family. Functional analysis indicated that SB-02 was able to hydrolyse the ginsenosides Rb1, Rc and Rd to F2 and C-K. Due to the conversion of ginsenosides, the strain's genome was sequenced and the genes were annotated by the NCBI. The average amino acid identity and average nucleotide identity values between SB-02 and the available reference genomes were 65.7 and 65.9 %, respectively. The novel isolate contained MK-7 as the predominant menaquinone, the major polyamine putrescine, and iso-C, iso-C G and iso-C 3-OH as major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Thus, based on the data presented here, strain SB-02 represents a novel species within a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is SB-02 (=KACC 21266=LMG 31707). The genome annotation of SB-02 shows many glycoside hydrolase genes, which may be responsible for the efficient production of many kinds of minor ginsenosides and will be very helpful for future research (target gene cloning) and mass production of either F2 or C-K.
主要人参皂苷向次要人参皂苷的转化因其生物和药学活性而备受关注。因此，对于人参皂苷的转化而言，寻找一种能产生新型活性糖苷水解酶的细菌菌株，对未来的研究以及次要人参皂苷的大规模生产具有重要意义。采集废水样本，使用里氏2A +七叶苷琼脂培养基筛选新型糖苷水解酶细菌菌株。结果，鉴定出一株新型糖苷水解酶阳性细菌菌株（SB - 02），并对其进行了多相分类分析。基于基因组分析，发现菌株SB - 02隶属于该科，与同科其他成员的序列相似性低于92.8%。功能分析表明，SB - 02能够将人参皂苷Rb1、Rc和Rd水解为F2和C - K。由于人参皂苷的转化，对该菌株的基因组进行了测序，并由美国国立生物技术信息中心（NCBI）对基因进行了注释。SB - 02与现有参考基因组之间的平均氨基酸同一性和平均核苷酸同一性值分别为65.7%和65.9%。该新型分离株以MK - 7作为主要甲基萘醌，以腐胺作为主要多胺，以异C、异C G和异C 3 - OH作为主要脂肪酸。主要极性脂质为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。因此，基于本文提供的数据，菌株SB - 02代表了该科一个新属内的一个新物种，为此提出新属名 ，新种名 。 的模式菌株为SB - 02（=KACC 21266 = LMG 31707）。SB - 02的基因组注释显示有许多糖苷水解酶基因，这可能是高效生产多种次要人参皂苷的原因，并且对未来的研究（目标基因克隆）以及F2或C - K的大规模生产将非常有帮助。
