Isolation and characterization of a novel glycoside hydrolase positive bacterial strain named sp. nov. with ginsenoside-converting activity.

A novel yellow-pigmented catalase- and oxidase-positive bacterial strain (designated NA20) was isolated from wetland soil and characterized. Results of 16S rRNA and draft genome sequence analysis placed strain NA20 within the genus of the family . Strain NA20 showed ≤97.1 % sequence similarity to members of the genus and the highest sequence similarity was found to DY (97.1%). The draft genome of strain NA20 had a total length of 7 144 125 base pairs. A total of 5659 genes were identified, of which 5613 were CDS and 46 RNA genes were assigned a putative function. Mining the genomes revealed the presence of 225 carbohydrate genes out of 1334 genes. Strain NA20 contained iso-C, iso-C G, iso-C 3-OH and summed feature 3 (C 7 and/or C 6) as major fatty acids. The predominant quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unknown polar lipid and one unknown aminophospholipid. Additionally, the functional analysis of NA20 showed the conversion of protopanaxatriol-mix type major ginsenosides (Rb1, Rc and Rd) to minor ginsenosides F2 and weak conversion of Rh2 and C-K within 24 h. As a result, the genotypic, phenotypic and taxonomic analyses support the affiliation of NA20 within the genus , for which the name sp. nov. is proposed. The type strain is NA20 (=KACC 22218=LMG 32198).