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分子印迹聚合物作为固相微萃取纤维用于从人血浆中分离选定抗生素

Molecularly Imprinted Polymers as Solid-Phase Microextraction Fibers for the Isolation of Selected Antibiotics from Human Plasma.

作者信息

Szultka-Młyńska Małgorzata, Janiszewska Daria, Buszewski Bogusław

机构信息

Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Torun, Poland.

Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Wilenska 4, 87-100 Torun, Poland.

出版信息

Materials (Basel). 2021 Aug 27;14(17):4886. doi: 10.3390/ma14174886.

DOI:10.3390/ma14174886
PMID:34500975
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8432719/
Abstract

The aim of this study was to examine the synthesis of novel molecularly imprinted polymer (MIP)-coated polythiophene and poly(3-methylthiophene) solid-phase microextraction fibers using the direct electropolymerization method. Synthesized SPME fibers were characterized with the use of various physicochemical instrumental techniques. MIP-SPME coatings were successfully applied to carry out the selective extraction of selected antibiotic drugs (amoxicillin, cefotaxime, metronidazole) and their metabolites (amoxycilloic acid, amoxicillin diketopiperazine, desacetyl cefotaxime, 3-desacetyl cefotaxime lactone, hydroxymetronidazole). Solid-phase microextraction parameters for the simultaneous determination and identification of target compounds were optimized using the central composite design (CCD), and they accounted for 5-15 min for desorption time, 3-10 for the pH of the desorption solvent, and 30-100 μL for the volume of the desorption solvent. High-performance liquid chromatography and mass spectrometry (MS) detectors such as quadrupole time-of-flight (Q-TOF MS) and triple quadrupole (QqQ MS) were applied to determine and to identify selected antibiotic drugs and their metabolites. The MIP-coated SPME are suitable for the selective extraction of target compounds in biological samples from patients in intensive care units.

摘要

本研究的目的是使用直接电聚合法研究新型分子印迹聚合物（MIP）包覆的聚噻吩和聚（3-甲基噻吩）固相微萃取纤维的合成。使用各种物理化学仪器技术对合成的固相微萃取纤维进行表征。MIP-固相微萃取涂层成功用于选择性萃取选定的抗生素药物（阿莫西林、头孢噻肟、甲硝唑）及其代谢物（阿莫西林酸、阿莫西林二酮哌嗪、去乙酰头孢噻肟、3-去乙酰头孢噻肟内酯、羟基甲硝唑）。使用中心复合设计（CCD）优化了同时测定和鉴定目标化合物的固相微萃取参数，解吸时间为5-15分钟，解吸溶剂的pH值为3-10，解吸溶剂的体积为30-100μL。应用高效液相色谱和质谱（MS）检测器，如四极杆飞行时间（Q-TOF MS）和三重四极杆（QqQ MS），来测定和鉴定选定的抗生素药物及其代谢物。MIP包覆的固相微萃取适用于从重症监护病房患者的生物样品中选择性萃取目标化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/f6ab84e586ff/materials-14-04886-g010a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/44f2eab6293b/materials-14-04886-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/9aa7fe49dda8/materials-14-04886-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/5acabc510ce6/materials-14-04886-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/cd7da659ce38/materials-14-04886-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/d65e1bffb85f/materials-14-04886-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/597fdb27734c/materials-14-04886-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/e44592023b98/materials-14-04886-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/649f8cda90e9/materials-14-04886-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/379a4c5fc2a5/materials-14-04886-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/f6ab84e586ff/materials-14-04886-g010a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/44f2eab6293b/materials-14-04886-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/9aa7fe49dda8/materials-14-04886-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/5acabc510ce6/materials-14-04886-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/cd7da659ce38/materials-14-04886-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/d65e1bffb85f/materials-14-04886-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/597fdb27734c/materials-14-04886-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/e44592023b98/materials-14-04886-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/649f8cda90e9/materials-14-04886-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/379a4c5fc2a5/materials-14-04886-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef48/8432719/f6ab84e586ff/materials-14-04886-g010a.jpg

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